Investigation of the correlation of allele polymorphism of renin-angiotensin system genes, nitric oxide synthase and folate cycle with the severity of ischemic stroke
- Authors: Levashova OA1, Baranova NI1, Zolkornyaev IG1
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Affiliations:
- Penza Institute for Further Training of Physicians
- Issue: Vol 99, No 3 (2018)
- Pages: 404-408
- Section: Theoretical and clinical medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/8891
- DOI: https://doi.org/10.17816/KMJ2018-404
- ID: 8891
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Abstract
Aim. To study the frequency of polymorphic gene variants encoding proteins of renin-angiotensin system [AGT Thr174Met (rs 4762), AGT Met235Thr (rs699), AGTR1 A1166C (rs5186)], endothelial factors [NOS3 C786T (rs2070744)], and folate cycle enzymes [MTHFR C677T (rs1801133)] in patients with various ischemic stroke severity.
Methods. 98 patients with ischemic stroke verified by magnetic resonance imaging and computed tomography scan of the brain, were examined. The severity of stroke was assessed by National Institutes of Health Stroke Scale. The allelic variants of genes were typified by polymerase chain reaction with the detection of amplification products in the «real time» mode.
Results. The analysis of genetic polymorphism frequency of renin-angiotensin system did not reveal statistically significant differences in the study groups. The polymorphism of NOSC786T gene in the study was also not associated with the severity of ischemic stroke. Among the studied polymorphic variants, only the C677T polymorphism of MTHFR gene was revealed to be reliably associated with severe course of acute cerebral ischemia.
Conclusion. C677T polymorphism of MTHFR gene is reliably associated with severe course of acute cerebral ischemia; carriage of 677T allele of MTHFR gene in the studied category of patients can cause an increased level of homocysteine and have an adverse effect on the course of acute cerebral ischemia.
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##article.viewOnOriginalSite##About the authors
O A Levashova
Penza Institute for Further Training of Physicians
Author for correspondence.
Email: olga.lewashowa@yandex.ru
Penza, Russia
N I Baranova
Penza Institute for Further Training of Physicians
Email: olga.lewashowa@yandex.ru
Penza, Russia
I G Zolkornyaev
Penza Institute for Further Training of Physicians
Email: olga.lewashowa@yandex.ru
Penza, Russia
References
- Gusev E.I., Martynov M.Yu., Kamchatnov P.R. Ischemic stroke. Current state of the problem. Doktor.ru. 2013; (5): 2–7. (In Russ)
- Vorob'ev P.A. Insul't. Normativnye dokumenty. (Stroke. Regulatory documents.) Moscow: N'yudiamed. 2010; 480 p. (In Russ.)
- Suslina Z.A. Neurology at the border of centuries: achievements and prospects. Vestnik RAMN. 2012; (8): 57–65. (In Russ.)
- Titov B.V., Matveeva N.A., Martynov M.Yu., Favorova O.O. Ischemic stroke as a complex polygenic disease. Molekulyarnaya biologiya. 2015; 49 (2): 224–248. (In Russ.)
- Strambovskaya N.N. Association of polymorphic genetic markers with various options of ischemic stroke. Journal of Siberian Medical Sciences. 2015; (1): 1–12. (In Russ.)
- Gayfullina F.G., Katmina M.N., Rizvanova F.F. et al. Role of genetic polymorphism in the pathogenesis of cerebrovascular disease. Kazanskiy meditsinskiy zhurnal. 2012; 93 (4): 663–667. (In Russ.)
- Korchagin V.I. Mironov K.O., Dribnokhodova O.P. et al. A role of genetic factors in the development of individual predisposition to ischemic stroke. Annaly klinicheskoy i eksperimental'noy nevrologii. 2016; 10 (1): 65–75. (In Russ.)
- Platunova I.M., Nikulina S.Yu., Cherkashina I.I. et al. Association of polymorphism RS699 angiotensinogen gene (AGT) with hemorrhagic and ischemic strokes. Sibirskoe meditsinskoe obozrenie. 2013; (3): 26–30. (In Russ.)
- Katsuya T., Morishita R. Gene polymorphism of angiotensin II type 1 and type 2 receptors. Curr. Pharm. Des. 2013; 19 (17): 2996–3001. doi: 10.2174/1381612811319170004.
- Zhang H., Sun M., Sun T. et al. Association between angiotensin II type 1 receptor gene polymorphisms and ischemic stroke: a meta-analysis. Cerebrovasc. Dis. 2011; 32 (5): 431–438. doi: 10.1159/000330655.
- Nakayama M., Yasue H., Yoshimura M. et al. T786C mutation in the 5ˊ-flankiring region of the endothelial nitric oxide synthase gene in associated with coronary spasm. Circulation. 1999; 99 (22): 2864–2870. doi: 10.1161/01.CIR.99.22.2864.
- Bushueva O.Yu., Stetskaya T.A., Korogodina T.V. et al. Gender-specific differences of endothelial nitric oxide synthase E298D polymorphism and the risk of stroke. Klinicheskaya meditsina. 2015; 93 (2): 34–40. (In Russ.)
- Kumar A., Misra S., Kumar P. et al. Association between endothelial nitric oxide synthase gene polymorphisms and risk of ischemic stroke: A meta-analysis. Neurol. India. 2017; 65 (1): 22–34. doi: 10.4103/0028-3886.198170.
- Spence J.D. Homocysteine lowering for stroke prevention: Unravelling the complexity of the evidence. Int. J. Stroke. 2016; 11 (7): 744–747. doi: 10.1177/1747493016662038.
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