Dependence of clot contraction (retraction) on the molecular and cellular blood composition
- Authors: Peshkova AD1, Lozhkin AP1, Fathullina LS2, Malyasev DV2, Bredikhin RA2, Litvinov RI1,3
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Affiliations:
- Kazan (Volga region) Federal University
- Interregional Clinical Diagnostic Center
- University of Pennsilvania
- Issue: Vol 97, No 1 (2016)
- Pages: 70-77
- Section: Experimental medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/2369
- DOI: https://doi.org/10.17750/KMJ2016-70
- ID: 2369
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Full Text
Abstract
Aim. To study the effect of the blood components on clot contraction dynamics in vitro.
Methods. The original method based on the optical detection of changes in the blood clot volume over time was used. Whole blood, as well as reconstructed samples using washed platelets, erythrocytes, purified fibrinogen, platelet-poor and platelet-rich plasma were studied.
Results. Blood clot contraction has a non-linear kinetics, reflecting the complexity of the underlying mechanisms. Platelets increase the blood clot contraction, while the red blood cells have an inhibitory effect. Blocking the fibrin and platelets interaction using the RGDS peptide, an integrin αIIbβ3 antagonist, reduces the extent and rate of clot contraction. The exogenous Ca2+ is not required for contraction, but its addition stabilizes clots by inhibiting the erythrocytes. Thrombin has a dose-dependent effect and increases the rate and extent of contraction. In blood samples of patients taking warfarin, blood clot contraction was delayed.
Conclusion. The blood clot contraction is a process which depends on many factors, including the blood cell composition, amount of fibrinogen, the endogenous thrombin activity and platelets interaction with fibrin; understanding the mechanisms of the blood clot contraction could form the basis for the development of novel approaches to the hemostatic disorders treatment.
Keywords
About the authors
A D Peshkova
Kazan (Volga region) Federal University
Author for correspondence.
Email: alinapeshkova@list.ru
A P Lozhkin
Kazan (Volga region) Federal University
Email: alinapeshkova@list.ru
L S Fathullina
Interregional Clinical Diagnostic Center
Email: alinapeshkova@list.ru
D V Malyasev
Interregional Clinical Diagnostic Center
Email: alinapeshkova@list.ru
R A Bredikhin
Interregional Clinical Diagnostic Center
Email: alinapeshkova@list.ru
R I Litvinov
Kazan (Volga region) Federal University; University of Pennsilvania
Email: alinapeshkova@list.ru
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