The effect of new quaternary phosphonium salts with highest alkyl substituents on the permeability of cell membranes for sodium ions in vitro
- Authors: Orlova OV1, Oslopov VN1, Sidullina SA1
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Affiliations:
- Kazan State Medical University, Kazan, Russia
- Issue: Vol 93, No 3 (2012)
- Pages: 505-507
- Section: Experimental medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/1879
- DOI: https://doi.org/10.17816/KMJ1879
- ID: 1879
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Abstract
Aim. To assess the effect of newly synthesized quaternary phosphonium salts with highest alkyl substituents (C10, C12, C14, C16, C18) on the permeability of cell membranes for sodium ions in vitro. Methods. In vitro studied was the effect of different concentrations of new quaternary phosphonium salts with highest alkyl substituents on the cell membrane permeability to sodium ions according to the rate of Na+-Li+-countertransport in the erythrocyte membrane. Results. Low concentration (0.001 mM) of substance C10 increased the rate of Na+-Li+-countertransport in the erythrocyte membrane by 11.9%, substance C12 - by 11.8%, substance C14 - by 12.7%, substance C16 - by 13%, substance C18 - by 12.3%. Higher concentrations of substances (0.01-0.05 mM) had no significant effect on the cell membrane permeability to sodium ions. Conclusion. An increase in the rate of Na+-Li+-countertransport in the erythrocyte membrane is influenced by minimal concentrations of a substance, under the influence of other concentrations the rate of Na+-Li+-countertransport doe not change.
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##article.viewOnOriginalSite##About the authors
O V Orlova
Kazan State Medical University, Kazan, Russia
Email: olqa570821@yandex.ru
V N Oslopov
Kazan State Medical University, Kazan, Russia
S A Sidullina
Kazan State Medical University, Kazan, Russia
References
- Агафонов А.А., Пиотровский В.К. Программа M-IND-оценки системных параметров фармакокинетики модельно-независимым методом статистических моментов // Хим.-фарм. ж. - 1991. - №10. - С. 16-19.
- Березовская И.В., Иванова В.М. Актуальные проблемы безопасности воспроизведённых лекарственных препаратов // Клин. исслед. лекарств. средств в России. - 2004. - №3-4. - С. 16-23.
- Егорова С.Н. Методы моделирования in vitro чрескожного всасывания лекарственных средств из лекарственных форм местного действия // Казан. мед. ж. - 2000. - №2. - С. 146-147.
- Постнов Ю.В., Орлов С.Н. Первичная гипертензия как патология клеточных мембран. - М.: Медицина, 1987. - 192 с.
- Bressole F., Bromet-Petit M., Audran M. Validation of liquid chromatographic and gas chromatographic methods. Applications to pharmacokinetics // J. Chromatogr. B. - 1996. - Vol. 686. - P. 3-10.
- Canessa M., Adragna N., Solomon H. et al. Increased sodium-lithium countertransport in red cells of patients with essential hypertension // New Engl. J. Med. - 1980. - Vol. 302. - P. 772-776.
- Karifes H.T., Shiu G., Shah V.P. Validation of bioanalytical methods // Pharmaceut. Research. - 1991. - Vol. 8. - P. 421-425.
- Shah V.P., Midha K.K., Shrihant D. et al. Analitical metods validation: bioavailability, bioequivalence and pharmacokinetic stadies. Conference report // Pharmaceut. Research. - 1992. - Vol. 9. - P. 588-592.
- Turck D., Busch U., Heinzel G., Narjes H. Clinical Pharmacokinetics of Meloxicam // Arzneim. Forsch. - 1997. - Vol. 3. - P. 253-258.
