Evaluation of a real-time nucleic acid sequencebased amplification (nasba) assay for diagnosis of urogenital chlamydial infection

Е. V. Shipitsyna; Шипицына Е. В.; А. М. Savicheva; Савичева А. М.; N. E. Vorobyova; Воробьева Н. Е.; Е. V. Sokolovsky; Соколовский Е. В.; А. Е. Guschin; Гущин А. Е.; Р. G. Ryzhikh; Рыжих П. Г.; G. A. Shipulin; Шипулин Г. А.; P. N. Krotin; Кротин П. Н.; L. V. Merculova; Меркулова Л. В.; О. Y. Landina; Ландина О. Ю.

doi:10.17816/JOWD83578

Evaluation of a real-time nucleic acid sequencebased amplification (nasba) assay for diagnosis of urogenital chlamydial infection

作者: Shipitsyna Е.V.¹, Savicheva А.М.¹, Vorobyova N.E.², Sokolovsky Е.V.², Guschin А.Е.³, Ryzhikh Р.G.³, Shipulin G.A.³, Krotin P.N.⁴, Merculova L.V.⁴, Landina О.Y.⁴
隶属关系:
1. Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
2. State Medical University named after academician I.P. Pavlova
3. FGUN Central Research Institute of Epidemiology, Rospotrebnadzor
4. Youth Center "Juventa"
期: 卷 54, 编号 4 (2005)
页面: 17-21
栏目: Original study articles
URL: https://journals.rcsi.science/jowd/article/view/83578
DOI: https://doi.org/10.17816/JOWD83578
ID: 83578

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This study was aimed to evaluate a new realtime nucleic acid sequence-based amplification (NASBA-Real- Time) assay for diagnosis of urogenital Chlamydia trachomatis infection. A total of 193 patients aged 16 to 42 (mean age, 22,8 years) were examined, with most of them having symptoms of urogenital infection. Cervical and urethral swabs from women and men, respectively, were investigated with the use of cell culture method, polymerase chain reaction (PCR) and NASBA- Real-Time. C. trachomatis infection was diagnosed in 29 patients (15 %): in 21 patients - by all the three methods, whereas 8 samples were culture negative. Sensitivity of PCR and NASBA- Real-Time methods were 100 %, cell culture method - 78,4 %. Negative predictive value of PCR and NASBA-Real-Time was found to be 100 %, and that of cell culture - 95,3 %. Specificity as well as positive predictive values of all the three methods equaled 100%. Thus, the new real-time NASBA-Real-Time assay was shown to be a very sensitive and specific test, which can be recommended as a confirmatory method for diagnosis of urogenital chlamydial infection.

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Chlamydia trachomatis, 16S rRNA, NAAT, PCR, NASBA-Real-Time

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Evaluation of a real-time nucleic acid sequencebased amplification (nasba) assay for diagnosis of urogenital chlamydial infection

全文:

详细

关键词

全文:

作者简介

Е. Shipitsyna

А. Savicheva

N. Vorobyova

Е. Sokolovsky

А. Guschin

Р. Ryzhikh

G. Shipulin

P. Krotin

L. Merculova

О. Landina

参考

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