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Post a Comment Creation of new pichia pastoris strains for recombinant protein production
- Authors: Muzaev D.M.1, Rumyantsev A.M.1, Sambuk E.V.1, Padkina M.V.1
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Affiliations:
- Saint Petersburg State University
- Issue: Vol 13, No 1 (2015)
- Pages: 10-15
- Section: Articles
- URL: https://journals.rcsi.science/ecolgenet/article/view/2401
- DOI: https://doi.org/10.17816/ecogen13110-15
- ID: 2401
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Abstract
Pichia pastoris yeasts are widely used as a production platform for heterologous proteins. Wide biotechnological use of these yeasts is determined by simplicity of cultivation, cheap media and ability to provide posttranslational modifications. Basic approaches for enhancement of the recombinant protein outcome constitue increasing number of gene copies, which encode target protein, as well as co-expression of supporting factors for protein folding, processing and secretion. Development of relevant plasmids and auxotrophic strains is essential for solving these tasks. In this study, we report plasmids and strains collection, whichwill allow to conduct integration of multiple foreign genes in P. pastoris genome
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##article.viewOnOriginalSite##About the authors
Dmitriy Mikhaylovich Muzaev
Saint Petersburg State University
Email: dmmuzaev@yandex.ru
master, Dept. Genetics and Biotechnology
Andrey Mikhaylovich Rumyantsev
Saint Petersburg State University
Email: rumyantsev-am@mail.ru
master, Dept. Genetics and Biotechnology
Elena Viktorovna Sambuk
Saint Petersburg State University
Email: esambuk@mail.ru
Dr. Sci. Biol, professor, Dept. Genetics and Biotechnology
Marina Vladimirovna Padkina
Saint Petersburg State University
Email: mpadkina@mail.ru
Dr. Sci. Biol, professor, Dept. Genetics and Biotechnology
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