Haplotypic diversity of mosquitoes of the genus Anopheles (Diptera, Culicidae) of North Vietnam

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Abstract

Background. Malaria in the territory of North Vietnam remains an actual problem. The species composition of mosquitoes of the genus Anopheles – the main vectors of malaria and the density of their populations – has a critical impact on the likelihood of the epidemic.

Materials and methods. We analyzed the variability of the BOLD fragment of the mitochondrial cox1 gene in a sampling set of Anopheles mosquitoes from the North Vietnam. Results. We found mosquitoes belonging to the species complexes: Anopheles sinensis, Anopheles vagus and Anopheles barbirostris. Nucleotide variability within the complexes does not exceed 2%. The differentiation into subspecies within the species complexes was not found.

Conclusion. The most common species of the genus Anopheles of North Vietnam is Anopheles sinensis — the main malaria vector. We predict the success of local transmission of malaria in this region in the case of malaria emergence.

About the authors

Thi Huong Vu

Moscow Region State University

Author for correspondence.
Email: Vuhuongmgou@gmail.com

Researcher

Russian Federation, 24, Very Voloshinoy street, Mytishi, 141014

Boris V. Andrianov

Vavilov Institute of General Genetics Russian Academy of Sciences

Email: andrianovb@mail.ru
ORCID iD: 0000-0002-0064-4696

PhD, Senior Researcher

Russian Federation, 3, Gubkin street, Moscow, 119991

Tatiana V. Gorelova

Vavilov Institute of General Genetics Russian Academy of Sciences

Email: tagor08@mail.ru

PhD, Researcher

Russian Federation, 3, Gubkin street, Moscow, 119991

Michail I. Gordeev

Moscow Region State University

Email: gordeev_mikhail@mail.ru

PhD, Head of the Department

Russian Federation, 24, Very Voloshinoy street, Mytishi, 141014

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Supplementary files

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2. Fig. 1. Cladistic analysis of mosquitoes of the genus Anopheles obtained on the basis of the analysis of the variability of a fragment of the cox1 gene, 649 bp in length . The names of the samples are given on the branches of the philogram. The Neighbor-Joining philogram was built using the (p-distance) method with the bootstrap support – 1000 replicas. The phylogram was constructed in MEGA 7. The sample collection locality is marked with a figure in front of the name: Laocai, Shonla, Yenbai, Kuangnin, Bakjiang. The Description of DNA samples of mosquitoes, see Table 1. The tree is constructed by the method (Neighbor-Joining). The bootstrap value of cluster support is shown next to the nodes of the phylogram. The tree is built in scale of the number of nucleotide replacements per site. The length of the control segment corresponds to 1% of nucleotide variability. Typical nucleotide sequences of the Anopheles of the hyrcanus group and Anopheles vagus species complex and Anopheles barbirostris species complex are given as controls. To mark these sequences are on the phylogram, the GenBank ID is given. The sequence of Drosophila yakuba cox1 of is taken as an external group

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3. Fig. 2. Median network of the mitochondrial haplotypes of An. cinensis, constructed in the program PopArt on the basis of the nucleotide polymorphism of the cox1 gene fragment, 649 bp in length. Mutations are indicated by strokes. The size of the circles is proportional to the number of synonymous haplotypes

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Copyright (c) 2018 Vu T., Andrianov B.V., Gorelova T.V., Gordeev M.I.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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