Reducing of LXRβ and PPARγ mRNA in M-CSF stimulated macrophages in patients with levels atherosclerosis

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Abstract

Nuclear receptors LXRα/β and PPARγ play an important role in lipid metabolism and reverse cholesterol transport. However, the influence of LXRα/β and PPARγ mRNA levels in macrophages on atherosclerosis remains unexplored. Using real time PCR, we determined LXRα mRNA, LXRβ mRNA and PPARγ mRNA levels in macrophages cultured for 5 days with macrophage colony-stimulating factor (M-CSF). Levels of LXRβ mRNA and PPARγ mRNA in patients with arterial stenosis were reduced when compared with the control group, p < 0.001. LXRa gene mRNA level in macrophages was not changed in the study groups, (p = 0.17). Thus, our study shows that the LXRβ and PPARγ genes expression levels in macrophages may be significant factors associated with the development of atherosclerosis.

About the authors

Yekaterina Petrovna Demina

B. P. Konstantinov Nuclear Physics Institute in Saint-Petersburg

Email: citritt@gmail.com
Molecular and Radiation Biophysics Department

Valentina Vadimovna Miroshnikova

B. P. Konstantinov Nuclear Physics Institute in Saint-Petersburg

Email: mutantropol@mail.ru
Molecular and Radiation Biophysics Department

Nikolay Vladimirovich Mayorov

Pavlov First Saint-Petersburg State Medical University

Email: nikolaj-majorov@yandex.ru
Hospital Surgery Department N 2

Vladimir Valentinovich Davydenko

Pavlov First Saint-Petersburg State Medical University

Email: kuzet@mail.ru
Hospital Surgery Department N 2

Alexander L`vovich Schwarzman

B. P. Konstantinov Nuclear Physics Institute in Saint-Petersburg

Email: aschwart1@yandex.ru
Molecular and Radiation Biophysics Department

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Copyright (c) 2014 Demina Y.P., Miroshnikova V.V., Mayorov N.V., Davydenko V.V., Schwarzman A.L.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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