SEARCHING FOR NEW FACTORS WHICH INFLUENCE TRANSLATION TERMINATION IN YEASTSACCHAROMYCES CEREVISIAE

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Abstract

In the yeast Saccharomyces cerevisiae translation termination factor eRF1 is encoded by the essential gene SUP45. Here we applied multicopy yeast library to identify a new factor which interacts with the release factor eRF1 in yeast Saccharomyces cerevisiae. We identified EСM23 gene whose overexpression decreased viability of sup45 nonsense mutants. We also showed that ECM23 overexpression had antisuppressor effect but the level of eRF1 protein was the same as that in the wild-type cells. The mechanisms by which ECM23 influence viability of sup45 mutants are discussed

About the authors

Svetlana Yevgenyevna Moskalenko

St. Petersburg Branch Vavilov Institute of General Genetics (RAS)

Email: smoskalenko@mail.ru
Ph. D., Senior Researcher, Assoc. Prof. Dept. of Genetics and Biotechnology

Olga Anatolyevna Murina

St.-Petersurg State University

Email: olga_murina@rambler.ru
student, Dept. of Genetics and Biotechnology

Olga Leonidovna Askinazi

St.-Petersurg State University

student, Dept. of Genetics and Biotechnology

Galina Anatolyevna Zhuravleva

St.-Petersurg State University

Email: zhouravleva@rambler.ru
Ph.D., Professor of the Dept. of Genetics and Biotechnology

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Copyright (c) 2013 Moskalenko S.Y., Murina O.A., Askinazi O.L., Zhuravleva G.A.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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