The use of R-nj, B1, Pl1 genes to improve marker properties in the selection of maize haploinducers
- Authors: Liu X.1, Ulyanov A.V.2, Khatefov E.B.2
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Affiliations:
- Maize Research Institute, Liaoning Academy of Agricultural Sciences
- N.I. Vavilov All-Russian Institute of Plant Genetic Resources
- Issue: Vol 20, No 3 (2022)
- Pages: 193-202
- Section: Genetic basis of ecosystems evolution
- URL: https://journals.rcsi.science/ecolgenet/article/view/108374
- DOI: https://doi.org/10.17816/ecogen108374
- ID: 108374
Cite item
Abstract
BACKGROUND: Studies were carried out on the creation of haploinductor lines using various methods for testing haploinductor and marker ability from the VIR maize genetic collection, adapted to the conditions of the Liaoning province in China and the South of Russia.
MATERIALS AND METHODS: On 22 maize testers, studies were carried out on the creation of haploinductor lines and testing the haploinductor and marker ability by the test-cross method. Sources of R1-nj, B1, Pl1 genes from the VIR maize genetic collection were used.
RESULTS: The lines of maize haploinducers Liaoyu No. 1, Liaoyu No. 2, Liaoyu No. 3 and Liaoyu No. 4 were created with a haploinduction frequency of 6.85%, 7.53%, 6.66% and 6.03%. respectively, with a high quality of labeling with the R1-nj, B1, Pl1 genes in test crosses.
CONCLUSIONS: In the process of selection of haploid maize on the basis of genes A, haploinductor lines with a high frequency of haploinduction and high efficiency of markers in test crosses, abundant pollen and grain productivity, resistance to late blight and lodging, and adaptation to the agroclimatic zone of Shenyang Province (China) were obtained and the North Caucasian Federal District (Russia).
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##article.viewOnOriginalSite##About the authors
Xinfang Liu
Maize Research Institute, Liaoning Academy of Agricultural Sciences
Email: xinfangliu2002@126.com
Master, Junior Research Associate
Taiwan, Province of China, Shenyang, LiaoningAlexey V. Ulyanov
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Email: krasnixin56@gmail.com
Research Laboratory Assistant
Russian Federation, Saint PetersburgEduard B. Khatefov
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Author for correspondence.
Email: haed1967@rambler.ru
ORCID iD: 0000-0001-5713-2328
SPIN-code: 7929-8148
Dr. Sci. (Biol.), Leading Research Associate
Russian Federation, Saint PetersburgReferences
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