Comparative analysis of the expression of stress-related genes in two pea genotypes contrasting in tolerance to cadmium

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Abstract

Background. A major problem of the environmental pollution with heavy metals, including cadmium, requires an intensive study of the molecular and genetic mechanisms underlying the tolerance of plants to these toxic substances. In this study we present a comparative analysis of the expression of stress-related genes in two pea genotypes contrasting in tolerance to cadmium.

Materials and methods. A unique mutant of pea SGECdt, characterized by the increased tolerance to cadmium, and initial line SGE were used. Gene expression was analyzed by Real Time PCR. Results. In the line SGE cadmium increase the expression of genes, encoding catalase, chitinase, chitinase-like protein PRP4A and dirigent protein PI206. In the mutant SGECdt cadmium increase the expression of genes, encoding chitinase, glutathione reductase and defensin DRR230. In control samples expression of genes encoding PRP4A and DRRR230 was enhanced in mutant SGECdt versus line SGE.

Conclusion. It was shown that, the reaction of the mutant SGECdt at the molecular level differs from that of the line SGE. In the mutant SGECdt, a change in the expression of a number of genes is observed, which may indicate that cadmium entering the cell causes activation of defense reactions.

About the authors

Olga A. Kulaeva

All-Russian Research Institute for Agricultural Microbiology

Author for correspondence.
Email: okulaeva@arriam.ru
ORCID iD: 0000-0003-2687-9693

PhD, Senior Scientist, Laboratory of Genetics of Plant-Microbe Interactions

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Emma S. Gribchenko

All-Russian Research Institute for Agricultural Microbiology

Email: gribemma@gmail.com

Technician, Laboratory of Genetics of Plant-Microbe Interactions

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Evgeny A. Zorin

All-Russian Research Institute for Agricultural Microbiology

Email: kjokkjok8@gmail.com

Technician, Laboratory of Genetics of Plant-Microbe Interactions

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Marina S. Kliukova

All-Russian Research Institute for Agricultural Microbiology

Email: marina.kliukova@gmail.com

Research Engineer, Laboratory of Genetics of Plant-Microbe Interactions

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Vladimir A. Zhukov

All-Russian Research Institute for Agricultural Microbiology

Email: vzhukov@arriam.ru

PhD, Head of the Lab, Laboratory of Genetics of Plant-Microbe Interactions

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

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2. Fig. 1. Changes in the expression of the PRP4A (A, Б), PI206 (В, Г), DRR230 (Д, Е) genes in the roots and shoots of the SGE line and SGECdt mutant under control conditions and after exposition with 3 μM CdCl2. “1 d.” – 1 day, “3 d.” – 3 days. * significant differences, p < 0.05. ** significant differences, p < 0.01. К – the root, П – shoot. “-” – control, “+” – treatment

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3. Fig. 2. Changes in the expression of the CAT (А, Б), CHT (В, Г), GR (Д, Е) genes in the roots and shoots of the SGE line and SGECdt mutant under control conditions and after exposition with 3 μM CdCl2. “1 d.” – 1 day, “3 d.” – 3 days. * significant differences, p < 0.05. ** significant differences, p < 0.01. К – the root, П – shoot. “-” – control, “+” – treatment

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4. Fig. 3. Changes in the expression of the PAL1 (А, Б), PAL2 (В, Г) genes in the roots and shoots of the SGE line and SGECdt mutant under control conditions and after exposition with 3 μM CdCl2. “1 d.” – 1 day, “3 d.” – 3 days. * significant differences, p < 0.05. ** significant differences, p <0.01. К – the root, П – shoot. “-” – control, “+” – treatment

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Copyright (c) 2018 Kulaeva O.A., Gribchenko E.S., Zorin E.A., Kliukova M.S., Zhukov V.A.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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