The possibilities of adapting the re-processing protocol in the practice of the ThinPrep Pap test usage

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Abstract

The method of liquid cytology is a newer diagnostic test compared to the traditional cytological examination of scrapings from the cervix and allows one to standardize the morphological examination and reduce the number of unsatisfactory samples. One of the factors determining the adequacy of the Pap test is the number of squamous epithelial cells in a sample. The abundance of blood elements, mucus, lubricant, or inflammatory elements may affect the informativeness of the sample. In the practice of the ThinPrep Pap test usage, when receiving an inadequate sample, a protocol for re-processing the contents of the vial is used, in order to neutralize the possible influence of such impurities on the final result. Here, we present a review on the methods of the re-processing protocol application and our own experience of adapting the protocol in the practice of a clinical diagnostic laboratory.

About the authors

Artem K. Aksamentov

Federal Scientific and Clinical Center for Specialized Medical Assistance and Medical Technologies of the Federal Medical Biological Agency

Author for correspondence.
Email: temaaxe@rambler.ru
ORCID iD: 0000-0002-7980-2537
SPIN-code: 7861-6556

MD

Russian Federation, 28 Orekhovy boulevard, 115682 Moscow

Nadezhda V. Melnikova

Federal Scientific and Clinical Center for Specialized Medical Assistance and Medical Technologies of the Federal Medical Biological Agency; Russian Scientific Center of Roentgenoradiology

Email: n_melnikova@list.ru
ORCID iD: 0000-0003-1193-352X
SPIN-code: 5050-8605

MD, PhD

Russian Federation, 28 Orekhovy boulevard, 115682 Moscow; Moscow

Eugenia V. Moshnina

Federal Scientific and Clinical Center for Specialized Medical Assistance and Medical Technologies of the Federal Medical Biological Agency

Email: moshnina.ev@fnkc-fmba.ru

биолог, клинико-диагностическая лаборатория

Russian Federation, 28 Orekhovy boulevard, 115682 Moscow

Nadezhda A. Kolyshkina

Federal Scientific and Clinical Center for Specialized Medical Assistance and Medical Technologies of the Federal Medical Biological Agency

Email: baklab_83@mail.ru
ORCID iD: 0000-0003-4486-5412

MD, PhD

Russian Federation, 28 Orekhovy boulevard, 115682 Moscow

Olga N. Kucherova

City Clinical Hospital named after V.V. Vinogradov

Email: ola-kucherova@mail.ru
ORCID iD: 0000-0003-3773-9629

MD, PhD

Russian Federation, Moscow

Vladimir P. Baklaushev

Federal Scientific and Clinical Center for Specialized Medical Assistance and Medical Technologies of the Federal Medical Biological Agency; Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences; Pulmonology Scientific Research Institute

Email: baklaushev.vp@fnkc-fmba.ru
ORCID iD: 0000-0003-1039-4245
SPIN-code: 3968-2971
https://fnkc-fmba.ru/about/komanda-upravleniya/

MD, PhD

Russian Federation, 28 Orekhovy boulevard, 115682 Moscow; Moscow; Moscow

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Supplementary files

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2. Fig. 1. Possibilities of the reprocessing protocol: а — a ready-made glass preparation with a halo effect and a small number of cellular elements; б — a ready-made preparation from the same material after using the reprocessing protocol (there is no halo effect, and the number of well-visualized cells allows one to accept the preparation as adequate).

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3. Fig. 2. Examples of the visual assessment scale (а) and samples (б): the sample on the left is ready for the preparation of the glass slide, the sample on the right requires additional processing before the preparation of the glass slide.

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Copyright (c) 2023 Aksamentov A.K., Melnikova N.V., Moshnina E.V., Kolyshkina N.A., Kucherova O.N., Baklaushev V.P.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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