DANIO RERIO (ZEBRAFISH) AS A MODEL FOR RADIOBIOLOGICAL RESEARCH

Objective: to estimate the possibility of using Danio rerio (Zebrafish) as a test system for assessing the effects of ionizing radiation using an erythrocyte micronuclei test. Materials and methods: 6 groups of fish (n = 120) were exposed to X-rays in doses of 0, 10, 20, 30, 40, 50 Gy to calculate semi-lethal dose (LD50 / 30). 9 groups of fish (n = 27) were exposed to X-ray at doses of 0 (control), 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 6,0, 8.0 Gy for constructing a dose-dependent curve of the occurrence of micronuclei in peripheral blood red blood cells. Results: the semi-lethal dose (LD50 / 30) for Danio rerio was calculated (24.9 Gy). The frequencies of micronuclei in the erythrocytes of Danio rerio were dose-dependent. Significant data of the frequency of occurrence of micronuclei were found when fish were irradiated at doses of 4.0, 6.0 and 8.0 Gy. Conclusion: Danio rerio fish are characterized by extreme radioresistance, thereby we suggest the prospect of their use in radiobiology as a lab test system for assessing the effectiveness of factors that induce mutagenic effects of ionizing radiation (1 figure, 1 table, bibliography: 11 refs).

Danio rerio, Zebrafish, bioindication, Danio rerio, in vivo model, model organism, radiobiological model, Zebrafish