VALIDATION EVALUATION OF METHODS FOR DETERMINING FLAVONOIDS IN BASILIC GRASS

Nowadays the study of medicinal herbs is still relevant due to its milder effect, less toxicity and ability not to cause addiction and allergies. The object of the study was the herb of the Ocimum basilicum which is widly used in folk medicine. It is known that it contains a variety of biologically active compounds: flavonoids, tannins, essential oil, anthocyanins, macro- and microelements, organic acids. Water extracts from Basil herb are used in folk medicine as an anti-inflammatory, antispasmodic, appetizing stimulant, tonic for problems of the gastrointestinal tract, upper respiratory tract, inflammatory diseases of the kidneys, etc. In order to use the Ocimum basilicum’s herb in official medicine, it is necessary to develop regulatory documentation that allows to evaluate its quality in accordance with the pharmacopeial requirements for medicinal herbs raw material. One of the many quality indicators of medicinal herbs raw materials is the quantitative content of the main biologically active compounds determines its pharmacological effect. We suppose, that flavonoids are such marker compounds for Ocimum basilicum’s herb. The paper presents the results of a validation assessment of the method for quantitative determination of the amount of flavonoids in the Ocimum basilicum’s herb, developed by differential spectrophotometry based on the reaction of complexation with aluminium chloride. As the validation characteristics of the developed methodology, the criteria of linearity, precision and accuracy are used.

basilica noble herb, validation assessment, quantitation, flavonoids, spectrophotometry