FIRST RESULTS OF EXPERIMENTAL TRANSPLANTATION OF LIMBAL EPITHELIAL STEM CELLS ON FIBRIN SCAFFOLD


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Abstract

Objective: is developing of method for transplanting cultured limbal epithelial stem cells on a fibrin scaffold with use of soft contact lens in case of limbal stem cells deficiency. Materials and methods. The cultured limbal epithelial stem cells were transplanted on fibrin scaffold. Transplantation of cultivated limbal stem cells deficiency on a fibrin scaffold with use of soft contact lens was performed to rabbit with limbal stem cells deficiency. Results of the study. The recovery of cornea was observed 8 days after limbal stem cells deficiency transplantation, the fibrin scaffold was completely resorbed. The conclusion. The use of a transplant of cultured limbal cells on a fibrin scaffold is a promising method for the subsequent successful keratoplasty in case of limbal stem cells deficiency (3 figs, 8 refs).

About the authors

V I Mikhailova

Cheboksary branch of the Academician S. Fyodorov Eye Microsurgery Federal State Institution

Cheboksary, Russia

A P Ponyatovskaya

Privolzhsky research medical university of the Ministry of health of the Russian Federation

Nizhny Novgorod, Russia

K A Alexandrova

Institute of advanced medical training Ministry of health of Chuvashia

Cheboksary, Russia

E N Bat’kov

Cheboksary branch of the Academician S. Fyodorov Eye Microsurgery Federal State Institution

Cheboksary, Russia

N A Pozdeyeva

Institute of advanced medical training Ministry of health of Chuvashia

Cheboksary, Russia

I V Mukhina

Lobachevsky University

Nizhny Novgorod, Russia

References

  1. Nieto-Miguel T., Calonge M., de la Mata A., López-Paniagua M., Galindo S., de la Paz M. F., Corrales R. M. A comparison of stem cell-related gene expression in the progenitor-rich limbal epithelium and the differentiating central corneal epithelium. Molecular Vision. 2011; 17: 2102-17.
  2. Dua H. S., Saini J. S., Azuara-Blanco A., Gupta P. Limbal stem cell deficiency: concept, aetiology, clinical presentation, diagnosis and management. Indian Journal of Ophthalmology. 2000; 48: 83-92.
  3. Sangwan V. S., Basu S., Vemuganti G. K., Sejpal K., Subramaniam S. V., Bandyopadhyay S., Krishnaiah S., Gaddipati S., Tiwari S., Balasubramanian D. Clinical outcomes of xeno-free autologous cultivated limbal epithelial transplantation: a 10-year study. British Journal of Ophthalmology. 2011; 95: 1525-9.
  4. Baradaran-Rafii A., Ebrahimi M., Kanavi M. R., Taghi-Abadi E., Aghdami N., Eslani M., Bakhtiari P., Einollahi B., Baharvand H., Javadi M. A. Midterm outcomes of autologous cultivated limbal stem cell transplantation with or without penetrating keratoplasty. Cornea. 2010; 29 (5): 502-9.
  5. Sejpal K., Ali M. H., Maddileti S., Basu S., Ramappa M., Kekunnaya R., Vemuganti G. K., Sangwan V. S. Cultivated Limbal Epithelial Transplantation in Children With Ocular Surface Burns. JAMA Ophthalmology. 2013; 131 (6): 731-6.
  6. Pauklin M., Fuchsluger T. A., Westekemper H., Steuhl K.-P., Meller D. Midterm results of cultivated autologous and allogeneic limbal epithelial transplantation in limbal stem cell deficiency. Developments of Ophthalmology. 2010; 45: 57-70.
  7. Hynds R. E., Bonfanti P., Janes S. M. Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond. EMBO Molecular Medicine. 2018; 10 (2): 139-50.
  8. Понятовская А. П., Коротченко С. А., Давыденко Д. В., Юдинцев А. В., Михайлова В. И., Шипунов А. А., Николаев И. А., Поздеева Н. А., Батьков Е. Н., Мухина И. В. Трансплантат лимбальных эпителиальных стволовых клеток на биорезорбируемом носителе. Современные технологии в медицине. 2017; 9 (4): 44-50.

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Copyright (c) 2018 Mikhailova V.I., Ponyatovskaya A.P., Alexandrova K.A., Bat’kov E.N., Pozdeyeva N.A., Mukhina I.V.

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