Email this article The effectiveness of the use of mesenchymal stromal cells for the treatment of lacerated wounds under conditions of hypothermia and hypoxia
- Authors: Volkova M.V.1, Boyarintsev V.V.1, Trofimenko A.V.1, Rybalkin S.P.2, Kovaleva E.V.2, Biryukov S.A.1, Fil'kov G.I.1, Durymanov M.O.1
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Affiliations:
- Moscow Institute of Physics and Technology (National Research University)
- Institute of Immunology of the Federal Medical and Biological Agency of Russia, Research Center for Toxicology and Hygienic Regulation of Biological Products
- Issue: Vol 41, No 3 (2022)
- Pages: 261-268
- Section: Original articles
- URL: https://journals.rcsi.science/RMMArep/article/view/109211
- DOI: https://doi.org/10.17816/rmmar109211
- ID: 109211
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Abstract
BACKGROUND: Patients Arctic conditions complicate the pathogenesis of various skin and soft tissue injuries. For the treatment of these diseases, the possibility of using multipotent mesenchymal stromal cells, which promote the proliferation of granular tissue cells, angiogenesis, and reduce the duration of the inflammatory phase during wound healing due to the secretion of cytokines and growth factors, is being considered.
AIM: In order to evaluate the effectiveness of cell therapy, experimental studies were carried out on the model of a lacerated wound in rats under conditions of hypoxia and hypothermia.
MATERIALS AND METHODS: The animals were kept in a climate chamber (15% oxygen, 4°C) for 48 hours. Injury was applied 24 hours after placement in controlled conditions. The introduction of stem cells was carried out a day after the wound was applied. Mesenchymal stromal cells obtained from the red bone marrow of Wistar rats were used for injection. The cell culture used had an immunophenotype corresponding to stem cells and had the ability to differentiate in the osteogenic, chondrogenic and adipogenic directions. During the study, the degree of inflammatory reaction in injured tissues and the presence of possible pathological discharges from the wound canal were assessed in rats, and the thickness of the injured paw was measured.
RESULTS: The stimulating effect of the suspension of mesenchymal stem cells on the dynamics of reducing the edema of the injured hip by 10% was established compared to the control group. To describe the process of inflammation, a histological analysis was performed on the 6th and 21st days after the wound was applied. On the 6th day of the study, a weak infiltration of lymphocytes in the muscle tissue was noted in rats that were injected with MMSC, which may indicate an earlier transition of the wound process to the proliferative phase.
CONCLUSION: The stimulating effect of the suspension of mesenchymal stem cells on the dynamics of reducing the edema of the injured hip by 10% was established compared to the control group.
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##article.viewOnOriginalSite##About the authors
Marina V. Volkova
Moscow Institute of Physics and Technology (National Research University)
Author for correspondence.
Email: biotech.volkova@list.ru
ORCID iD: 0000-0001-5966-3026
SPIN-code: 4104-5195
Junior Research Fellow
Russian Federation, Dolgoprudny, Moscow RegionValery V. Boyarintsev
Moscow Institute of Physics and Technology (National Research University)
Email: marinarage@mail.ru
ORCID iD: 0000-0001-9707-3262
SPIN-code: 2491-7199
докт. мед. наук, профессор, главный научный сотрудник
Russian Federation, Dolgoprudny, Moscow RegionAlexander V. Trofimenko
Moscow Institute of Physics and Technology (National Research University)
Email: trofimenko.av@mipt.ru
SPIN-code: 5228-7073
M.D., Ph.D. (Medicine), Associate Professor, Head of Laboratory
Russian Federation, Dolgoprudny, Moscow RegionSergey P. Rybalkin
Institute of Immunology of the Federal Medical and Biological Agency of Russia, Research Center for Toxicology and Hygienic Regulation of Biological Products
Email: rybalkin-sp@yandex.ru
ORCID iD: 0000-0002-2933-5758
SPIN-code: 1988-4621
Ph.D. (Biology), director
Russian Federation, Serpukhov City District, Moscow RegionElena V. Kovaleva
Institute of Immunology of the Federal Medical and Biological Agency of Russia, Research Center for Toxicology and Hygienic Regulation of Biological Products
Email: e-kovaleva@yandex.ru
SPIN-code: 2952-1038
Senior Researcher
Russian Federation, Serpukhov City District, Moscow RegionStanislav A. Biryukov
Moscow Institute of Physics and Technology (National Research University)
Email: biryukov.sa@mipt.ru
D.Sc. (Physics and Mathematics), Senior Researcher
Russian Federation, Dolgoprudny, Moscow RegionGleb I. Fil'kov
Moscow Institute of Physics and Technology (National Research University)
Email: filcom.gl@gmail.com
SPIN-code: 7733-8460
Researcher
Russian Federation, Dolgoprudny, Moscow RegionMichail O. Durymanov
Moscow Institute of Physics and Technology (National Research University)
Email: durymanov.mo@mipt.ru
SPIN-code: 5796-0022
ResearcherId: T-8711-2018
Ph.D. (Biology), Deputy Head of Laboratory
Russian Federation, Dolgoprudny, Moscow RegionReferences
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