Vol 6, No 3 (2024)

The work is devoted to research on the selection of peptone for the optimization of the nutrient medium for the antimicrobial activity by the agar diffusion method of neomycin (framycetin), which have a similar chemical structure. The paper substantiates the relevance and importance of developing a formulation, selecting the optimal composition (ingredients), the quantitative ratio of ingredients, the pH of the medium, compliance of the proposed nutrient media with the State Pharmacopoeia of the Russian Federation and the European Pharmacopoeia for the standardization of antibiotics. The aim of the work was to study the components of the nutrient medium in comparison with the pharmacopoeial standards recommended for determining the effectiveness of antibiotics. It is shown that for carrying out microbiological tests in the standardization of antimicrobial drugs, the nutrient medium is the main one, and the correctness and accuracy of the interpretation of the research results depend on their quality. The experiment studied four brands of dry peptone and broth from different manufacturers – LLC NITSF and FBUN GNTs PMB (Obolensk) when working with the test strain of the spore culture Bacillus subtilis ATCC 6633. An optimal nutrient medium is proposed, the composition and conditions of its preparation are selected. To determine the antimicrobial activity of framycetin by the agar diffusion method, a developed medium for determining the effectiveness of neomycin when working with the spore-forming form of the test strain Baccillus subtillis ATCC 6633 is proposed.

The study focuses on pancreatic cancer (C25), a malignant neoplasm arising from the ductal epithelium or glandular tissue of the pancreas, which accounts for 3% of the overall cancer incidence in Russia. The rates of incidence and mortality are rising, with the number of newly registered patients in Russia being lower than the number of recorded deaths, although the one-year mortality rate among patients is decreasing. The direct cause of pancreatic cancer (C25) remains unknown. Risk factors for developing this condition include genetics, smoking, alcohol abuse, a sedentary lifestyle, excessive meat consumption, and a lack of plant-based foods in the diet. Diseases associated with a high risk of this pathology include long-standing chronic pancreatitis, newly diagnosed type 2 diabetes in individuals aged 50-70, and cystic neoplasms of the pancreas. The aim of the study is to examine the patterns of incidence and mortality, the quality of patient registration, the characteristics of tumor localization, and the histological structure of pancreatic cancer (C25). The study will also track the nature of annual mortality among patients and explore the possibilities, methods, and techniques of modern diagnosis and treatment for these patients. The research confirmed the significant burden of pancreatic cancer (C25), primarily due to the late detection of this disease. The study provided sufficient material to analyze age-related risk factors, as well as the patterns of incidence and mortality in the population. It also examined and presented mortality rates among patients, including during the first year of observation.

Updates of the eukaryotic system (domain Eukarya) in the interface “Eukaryotic supergroups: Taxonomy/Biotechnology interface” are described. The nine-kingdom system Eukarya has the following form: subdomain Excavata (kingdoms Archezoa, Discoba), subdomain Obimoda (kingdoms Crumalia, Amoebozoa, Obazoa), subdomain Corticata (kingdoms Eocorticata, Haptista, Tsaralia, Plantae incl. Cryptista, Glaucophyta, Rhodophyta, Chloroplastida).

The biographical sketch is dedicated to Professor Oleg Nikolaevich Trofimov (1927–2013), PhD in Pedagogic Sciences, who headed the Department of Physical Education at the Leningrad Institute Chemical Pharmaceuticals from 1973 to 1995. The main stages of O. N. Trofimov’s professional activity corresponded to the general context of the development of priority areas of physical education in the system of secondary and higher education. O. N. Trofimov skillfully combined managerial activities with research and educational-methodological work. He made a significant contribution to the development of the health-improving direction of physical education at the university, to the organization and conduct of research aimed at solving the problems of improving health and increasing the physical fitness of students. The scientific activity of O. N. Trofimov was devoted to the theory and methods of physical education, issues of physical development, the development of comprehensive training programs in vocational-applied physical training and additional public specialty within the framework of the Faculty of Public Professions. The results of the research carried out at the department were widely introduced into the educational process of physical education and served as the basis for the development of innovative technologies for constructing the educational process in the discipline “Elective physical culture: recreational physical education”, as well as the program for the development of physical culture and sports, the formation of a healthy lifestyle at the Saint Petersburg Chemical and Pharmaceutical University. O. N. Trofimov was a war veteran, awarded government awards, including the medal “For the Defense of Leningrad”. The essay uses archival data, bibliographic publications, specialized journals and collections of materials from scientific and methodological conferences, materials from the department and the personal archives of O. N. Trofimov.