Email this article Analysis of the effectiveness of a new antimicrobial agent for disinfection in veterinary practice
- Authors: Bukovskaya J.A.1, Chernykh T.F.1, Zharikov M.V.1
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Affiliations:
- Saint Petersburg State Chemical and Pharmaceutical University
- Issue: Vol 6, No 1 (2024)
- Pages: 10-15
- Section: Pharmaceutical Sciences
- URL: https://journals.rcsi.science/PharmForm/article/view/256780
- DOI: https://doi.org/10.17816/phf630301
- ID: 256780
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Abstract
The loss of effectiveness of standard treatment methods and the increased risk of rapid spread of infections are serious consequences of this problem. This underscores the need to develop new approaches to combating pathogen resistance and ensuring effective control of infections. Studies of antimicrobial activity were conducted on strains of C. albicans, A. niger, and B. subtilis. The minimal fungicidal concentration was determined based on the growth of microorganisms C. albicans, A. niger on Sabouraud agar media containing different concentrations of a new substance – a derivative of thiadiazole. The sporicidal activity was determined by culturing the test culture B. subtilis in meat-peptone broth, followed by seeding on meat-peptone agar. Sporicidal activity was determined by inoculating the test culture of B. subtilis into meat-peptone broth, followed by plating on meat-peptone agar. Control assays were performed by inoculating the test cultures on corresponding media without the test substances. The new disinfectant – a derivative of thiadiazole – demonstrated high antimicrobial activity. The aim of this research was to study the new chemical substance for antimicrobial (antifungal and sporicidal) activity and the creation of new types of biocides. The studies showed that the minimum fungicidal concentration of the 0.3% solution in a 30% alcohol solution against C. albicans RCPGY401 was 1.56 ± 1.06 μg/mL, A. niger 137a – 1.56 ± 1.29 μg/mL. Furthermore, the material exhibited sporicidal activity against B. subtilis ATCC 10702, with an effective concentration of – 3.12 ± 1.34 μg/mL. The research confirms the activity of the new compound against the studied microorganisms and allows for its recommendation for further study (treatment regimens, safety, control methods, etc.) for use in veterinary practice for various treatment purposes.
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##article.viewOnOriginalSite##About the authors
Julia A. Bukovskaya
Saint Petersburg State Chemical and Pharmaceutical University
Author for correspondence.
Email: ybukovskaya@mail.ru
Applicant for the Department of Microbiology
Russian Federation, Saint PetersburgTatiana F. Chernykh
Saint Petersburg State Chemical and Pharmaceutical University
Email: tatiana.odegova@pharminnotech.com
D.Sc. in Pharmaceutical Sciences, Professor, Head of the Department of Microbiology
Russian Federation, Saint PetersburgMikhail V. Zharikov
Saint Petersburg State Chemical and Pharmaceutical University
Email: zharikov.mihail@pharminnotech.com
Senior Laboratory Assistant of Industrial Ecology
Russian Federation, Saint PetersburgReferences
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