Analysis of the effectiveness of a new antimicrobial agent for disinfection in veterinary practice

封面

如何引用文章

全文:

详细

The loss of effectiveness of standard treatment methods and the increased risk of rapid spread of infections are serious consequences of this problem. This underscores the need to develop new approaches to combating pathogen resistance and ensuring effective control of infections. Studies of antimicrobial activity were conducted on strains of C. albicans, A. niger, and B. subtilis. The minimal fungicidal concentration was determined based on the growth of microorganisms C. albicans, A. niger on Sabouraud agar media containing different concentrations of a new substance – a derivative of thiadiazole. The sporicidal activity was determined by culturing the test culture B. subtilis in meat-peptone broth, followed by seeding on meat-peptone agar. Sporicidal activity was determined by inoculating the test culture of B. subtilis into meat-peptone broth, followed by plating on meat-peptone agar. Control assays were performed by inoculating the test cultures on corresponding media without the test substances. The new disinfectant – a derivative of thiadiazole – demonstrated high antimicrobial activity. The aim of this research was to study the new chemical substance for antimicrobial (antifungal and sporicidal) activity and the creation of new types of biocides. The studies showed that the minimum fungicidal concentration of the 0.3% solution in a 30% alcohol solution against C. albicans RCPGY401 was 1.56 ± 1.06 μg/mL, A. niger 137a – 1.56 ± 1.29 μg/mL. Furthermore, the material exhibited sporicidal activity against B. subtilis ATCC 10702, with an effective concentration of – 3.12 ± 1.34 μg/mL. The research confirms the activity of the new compound against the studied microorganisms and allows for its recommendation for further study (treatment regimens, safety, control methods, etc.) for use in veterinary practice for various treatment purposes.

作者简介

Julia Bukovskaya

Saint Petersburg State Chemical and Pharmaceutical University

编辑信件的主要联系方式.
Email: ybukovskaya@mail.ru

Applicant for the Department of Microbiology

俄罗斯联邦, Saint Petersburg

Tatiana Chernykh

Saint Petersburg State Chemical and Pharmaceutical University

Email: tatiana.odegova@pharminnotech.com

D.Sc. in Pharmaceutical Sciences, Professor, Head of the Department of Microbiology

俄罗斯联邦, Saint Petersburg

Mikhail Zharikov

Saint Petersburg State Chemical and Pharmaceutical University

Email: zharikov.mihail@pharminnotech.com

Senior Laboratory Assistant of Industrial Ecology

俄罗斯联邦, Saint Petersburg

参考

  1. Osnovy obshchei epizootologii: Uchebnoe posobie dlya studentov vuzov po spets. “Veterinariya” / ed. I. A. Bakulov and A.S. Donchenko. – Novosibirsk, 2008. – 263 p. (In Russ.).
  2. European Food Safety Authority; European Centre for Disease Prevention and Control. The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2018/2019. EFSA J. 2021;19(4):e06490. doi: 10.2903/j.efsa.2021.6490
  3. O’Neill J. Review on Antimicrobial Resistance Antimicrobial Resistance: Tackling a crisis for the health and wealth of nations. London: Review on Antimicrobial Resistance. 2014. [Elektronnyi resurs] – URL: Available from: https://amr-review.org/sites/default/files/AMR%20Review%20Paper%20-%20Tackling%20a%20crisis%20for%20the%20health%20and%20wealth%20of%20nations_1.pdf
  4. O’Neill J. Tackling drug-resistant infections globally: final report and recommendations. London: The Review on Antimicrobial Resistance; 2016. [Elektronnyi resurs] – URL: https://amr-review.org/sites/default/files/160518_Final%20paper_with%20cover.pdf.
  5. de Kraker ME, Stewardson AJ, Harbarth S. Will 10 Million People Die a Year due to Antimicrobial Resistance by 2050? PLoS Med. 2016 Nov 29;13(11):e1002184. doi: 10.1371/journal.pmed.1002184.
  6. Hansson K, Brenthel A. Imagining a post-antibiotic era: a cultural analysis of crisis and antibiotic resistance. Med Humanit. 2022 Sep;48(3):381–388. doi: 10.1136/medhum-2022-012409.
  7. Antimicrobial Resistance Collaborators. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet 2022;399:629–55 Published Online January 20, 2022.
  8. Danilov A. I. Antimicrobial resistance: arguments and facts / A. I. Danilov, L. P. Zharkova // Clinical pharmacology and therapy. – 2017. – T. 26, No. 5. – P. 6–9. (In Russ.).
  9. Bellini C., Troilet N. Résistance aux antibiotiques: état des lieux en Europe et en Suisse et impact pour le praticien = Antibiotic resistance: situation in Europe and Switzerland, and impact for the physician. Rev. Med. Suisse. 2016; 12 (534): 1699–1702. PMID: 28686394. (in French).
  10. Review on Antimicrobial Resistance. Antimicrobial Resistance: Tackling a Crisis for the Health and Wealth of Nations. 2014. [Elektronnyi resurs] – URL: https://amr-review.org/sites/default/files/AMR%20Review%20Paper%20-%20Tackling%20a%20crisis%20for%20the%20health%20and%20wealth%20of%20nations_1.pdf
  11. Frolova V. V. Antimicrobial activity of new derivatives of 1,3,4-thiadiazole / V. V. Frolova, S. V. Gurina, I. P. Yakovlev, V. N. Yuskovets // Modern medicine: current issues and development prospects: Collection of scientific papers on results of the international scientific and practical conference, Ufa, September 11, 2017. Volume Issue IV. – Ufa: Innovation Center for the Development of Education and Science, 2017. – P. 62–64. (In Russ.)
  12. Kulitsa M. M. Modern disinfectants as a tool for managing the epizootic situation / M. M. Kulitsa, M. I. Dronfort // Veterinary medicine and feeding. – 2019. – No. 2. – P. 32–33. – doi: 10.30917/ATT-VK-1814-9588-2019-2-12. (In Russ.)
  13. Shilova I. V. Effektivnost’ primeneniya novykh dezinfitsiruyushchikh sredstv v veterinarii / E. N. Shilova, I. V. Vyalykh, D. M. Kadochnikov, O. G. Subbotina // Agrarian Bulletin of the Urals. – 2013. – No. 8(114). – P. 9–11. (In Russ.).
  14. Rukovodstvo R4.2.2643-10 “Metody laboratornykh issledovanii i ispytanii dezinfektsionnykh sredstv dlya otsenki ikh effektivnosti i bezopasnosti” (utv. Federal’noi sluzhboi po nadzoru v sfere zashchity prav potrebitelei i blagopoluchiya cheloveka, Glavnym gosudarstvennym sanitarnym vrachom RF 1 iyunya 2010 g.) (s izmeneniyami i dopolneniyami) / [Elektronnyi resurs] // GARANT: [sait]. – URL: https://base.garant.ru/4192979/. (In Russ.).
  15. Odegova T. F. Some aspects of the use of disinfectants in pharmaceutical enterprises and in microbiological laboratories that control the quality of medicines / T. F. Odegova, O. V. Gunar, N. A. Melnikova [etc.] // Perm Medical Journal. – 2011. – T. 28, No. 1. – P. 135–139. (In Russ.).
  16. Kasatkin, A. S. Application of disinfectants in the food industry / A. S. Kasatkin // Food industry. – 2020. – No. 2(44). – pp. 54–57. (In Russ.).
  17. Shcherbakov P. N. Study of the antimicrobial activity of new generation disinfectants / P. N. Shcherbakov, K. V. Stepanova, E. N. Barzanova // Prospects for the development of veterinary science and its role in ensuring food safety: collection of materials of the international scientific conference, dedicated to the 95th anniversary of the founding of the Veterinary Research Institute, Tashkent, May 21, 2022 / State Committee for Veterinary Medicine and Livestock Development of the Republic of Uzbekistan, Veterinary Research Institute. Volume 1. – Tashkent: Global Book Publishing Services, 2022. – P. 295–299.
  18. Sharafutdinova V. I. Problems of choosing disinfectants in medical organizations at the present stage and solutions / V. I. Sharafutdinova // Health Architecture. – 2022. – No. 1. – P. 29–41. (In Russ.).
  19. Kanishchev V. V. Some scientific and practical aspects of the use of disinfectants in the practice of healthcare facilities / V. V. Kanishchev, N. I. Eremeeva // Polyclinic. – 2013. – No. 4–2. – P. 104–110.
  20. Goloshva E. V. Monitoring the effectiveness of modern disinfectants used in one of the hospitals in Rostov-on-Don / E. V. Goloshva, K. G. Markova, A. V. Aleshukina [etc.] // Chief Doctor of the South of Russia. – 2023. – No. 1(87). – P. 52–55.

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Eco-Vector, 2024

Creative Commons License
此作品已接受知识共享署名-非商业性使用-禁止演绎 4.0国际许可协议的许可。
 


Согласие на обработку персональных данных

 

Используя сайт https://journals.rcsi.science, я (далее – «Пользователь» или «Субъект персональных данных») даю согласие на обработку персональных данных на этом сайте (текст Согласия) и на обработку персональных данных с помощью сервиса «Яндекс.Метрика» (текст Согласия).