The influence of short-term heating on the activity of amylolytic enzymes in honey

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Abstract

This study presents the results of the analysis of the activity of amylolytic enzymes in honey, depending on short-term heating, simulating the process of adding honey to hot tea. Additionally, a comparison of this activity with similar enzymes present in the pharmaceutical preparation "Festal" was conducted. Regularities in the change of amylolytic enzyme activity were established, contributing to the development of recommendations for honey consumption considering this parameter. The obtained results demonstrate significantly lower activity of amylolytic enzymes in honey compared to the pharmaceutical preparation. However, considering differences in consumption, it is possible to assess them as comparable. It was also revealed that the activity of amylolytic enzymes in honey changes during short-term heating, not decreasing until the temperature reaches 30°C. Based on the in vitro activity analysis, the tested sample corresponds to the pharmaceutical preparation "Festal," allowing for prospects for further research into the impact of honey's amylolytic enzymes on the human body, requiring more precise methods of study. 

About the authors

Varvara V. Solovyova

Saint Petersburg State Chemical and Pharmaceutical University of the Ministry of Health of the Russian Federation

Email: solovyova.varvara@spcpu.ru

3rd year student of the Faculty of Pharmacy

Russian Federation, Saint Petersburg

Vyacheslav A. Tikhomirov

Saint Petersburg State Chemical and Pharmaceutical University of the Ministry of Health of the Russian Federation

Author for correspondence.
Email: vyacheslav.tihomirov@spcpu.ru

3rd year student of the Faculty of Pharmacy

Russian Federation, Saint Petersburg

Ksenia A. Filatova

Saint Petersburg State Chemical and Pharmaceutical University of the Ministry of Health of the Russian Federation

Email: kseniya.filatova@spcpu.ru

3rd year student of the Faculty of Pharmacy

Russian Federation, Saint Petersburg

Sergey G. Paramonov

Saint Petersburg State Chemical and Pharmaceutical University of the Ministry of Health of the Russian Federation

Email: sergei.paramonov@pharminnotech.com
ORCID iD: 0000-0003-3016-9010
SPIN-code: 1302-0720
ResearcherId: JPK-6124-2023

Ph.D. in Biological Sciences, Associate Professor at the Industrial Ecology Department

Russian Federation, Saint Petersburg

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Supplementary files

Supplementary Files
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1. JATS XML
2. Fig. 1. Dependence of enzyme activity on the temperature of short-term heating of honey

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