Email this article Potential hepatoprotective properties of synthetic derivatives of thiourea — 2-aminobenzothiazole and IEM-2320
- Authors: Klimenko D.I.1, Demidova E.O.2, Asatryan T.T.1, Myznikov L.V.3, Nadbitova N.D.3, Matveeva E.V.1, Moskvicheva S.V.1, Borzykh S.A.1, Evdokimova N.R.3, Lukk M.V.4, Ramsh S.M.5, Ganapolsky V.P.4, Karpova I.V.3
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Affiliations:
- North-Western State Medical University named after I.I. Mechnikov
- Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency of Russia
- Institute of Experimental Medicine
- Kirov Military Medical Academy
- Saint Petersburg State Institute of Technology (Technical University)
- Issue: Vol 24, No 2 (2024)
- Pages: 69-78
- Section: Original research
- URL: https://journals.rcsi.science/MAJ/article/view/271133
- DOI: https://doi.org/10.17816/MAJ630414
- ID: 271133
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Abstract
BACKGROUND: Toxic hepatitis is characterized by a significant severity of the course and by high mortality. So, search for effective means of correcting this condition seems to be relevant. It has previously been shown that some thiazolo[5,4-b]indole derivatives are capable to prevent experimental toxic liver damage in laboratory animals. In continuation of this direction, the study of the hepatoprotective properties of synthetic derivatives of thiourea 2-aminobenzothiazole and IEM-2320 was undertaken.
AIM: To study the potential hepatoprotective properties of 2-aminobenzothiazole and IEM-2320 in acute carbon tetrachloride poisoning.
MATERIALS AND METHODS: Experiments were carried out on 30 male white outbred rats (150–270 g). On a model of carbon tetrachloride poisoning (50% solution of CCl4 in vaseline oil, 0.2 ml/100 g of body weight), the potential hepatoprotective properties of 2-aminobenzothiazole (32 mg/kg) and IEM-2320 (20 mg/kg) were investigated. Ademethionine (Heptral, AbbVie, S.r.L, Italy) at a dose of 20 mg/kg was used as a comparison drug. The studied drugs were administered intraperitoneally 4 times: one hour before the introduction of CCl4, and then — for three subsequent days after the poisoning with CCl4. The results were compared with those of 2 control groups. Animals of the 1st control group were not exposed to CCl4, instead of the studied drugs, they were injected with an equivalent volume of saline solution (0.2 ml/100 g of body weight). Animals of the 2nd control group did not receive any treatment after the CCl4 poisoning. Blood was taken on the seventh day of the experiment. The total contents of protein, glucose, and total cholesterol in blood serum were determined, and the activities of alanine aminotransferase and aspartate aminotransferase were evaluated.
RESULTS: Under the CCl4 poisoning, the administration of 2-aminobenzothiazole led to a significant decrease in the concentrations of glucose, total protein, total cholesterol and alanine aminotransferase activity. When IEM-2320 was administered under the condition of poisoning, a decrease in the concentrations of total protein, glucose, urea, total cholesterol, and the alanine aminotransferase activity was observed.
CONCLUSIONS: A significant decrease in the biochemical parameters of blood serum after administration of 2-aminobenzothiazole and IEM-2320 under the condition of CCl4 poisoning demonstrates thir potential hepatoprotective properties under acute hepatotropic poisonings.
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##article.viewOnOriginalSite##About the authors
Dmitry I. Klimenko
North-Western State Medical University named after I.I. Mechnikov
Author for correspondence.
Email: dima.klimenko999@mail.ru
ORCID iD: 0009-0007-8168-7228
SPIN-code: 8481-4489
5th-Year Student
Russian Federation, Saint PetersburgEkaterina O. Demidova
Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency of Russia
Email: bedskaya.667@yandex.com
ORCID iD: 0009-0003-0820-8471
SPIN-code: 1618-1510
2nd-Year Resident, Junior Researcher at the Laboratory of Morphology and Electron Microscopy
Russian Federation, Saint PetersburgTatevik T. Asatryan
North-Western State Medical University named after I.I. Mechnikov
Email: tatevik.asatryan@szgmu.ru
ORCID iD: 0000-0002-9146-3080
SPIN-code: 5587-1360
MD, Cand. Sci. (Medicine), Associate Professor of the Department of Clinical Laboratory Diagnostics, Biological and General Chemistry named after V.V. Sokolovsky, Head of the Central Research Laboratory
Russian Federation, Saint PetersburgLeonid V. Myznikov
Institute of Experimental Medicine
Email: postleo@mail.ru
ORCID iD: 0000-0002-0863-3027
Dr. Sci. (Chemistry), Researcher of the Department of Neuropharmacology named after S.V. Anichkov
Russian Federation, Saint PetersburgNatalia D. Nadbitova
Institute of Experimental Medicine
Email: natali_805@mail.ru
ORCID iD: 0000-0002-2957-226X
SPIN-code: 4153-1270
MD, Cand. Sci. (Medicine), Researcher of the Department of Neuropharmacology named after S.V. Anichkov
Russian Federation, Saint PetersburgEkaterina V. Matveeva
North-Western State Medical University named after I.I. Mechnikov
Email: lika.hell@mail.ru
ORCID iD: 0009-0006-0695-6400
5th-Year Student
Russian Federation, Saint PetersburgSofia V. Moskvicheva
North-Western State Medical University named after I.I. Mechnikov
Email: moskvichevasofia@yandex.ru
ORCID iD: 0009-0007-6058-2945
3rd-Year Student
Russian Federation, Saint PetersburgSofia A. Borzykh
North-Western State Medical University named after I.I. Mechnikov
Email: borzih.s2002@gmail.com
ORCID iD: 0009-0002-6882-8421
4th-Year Student, Laboratory Assistant Researcher at the Central Research Laboratory
Russian Federation, Saint PetersburgNatalia R. Evdokimova
Institute of Experimental Medicine
Email: enatalyar@mail.ru
ORCID iD: 0000-0003-4318-2556
Cand. Sci. (Biology), Researcher of the Department of Neuropharmacology named after S.V. Anichkov
Russian Federation, Saint PetersburgMargarita V. Lukk
Kirov Military Medical Academy
Email: m_lukk@mail.ru
ORCID iD: 0009-0003-0853-7769
SPIN-code: 5125-6277
MD, Cand. Sci. (Medicine), Associate Professor of the Department of Pharmacology
Russian Federation, Saint PetersburgStanislav M. Ramsh
Saint Petersburg State Institute of Technology (Technical University)
Email: sramsh@technolog.edu.ru
ORCID iD: 0000-0003-4613-068X
SPIN-code: 6777-2246
Dr. Sci. (Chemistry), Professor, Head of the Department of Chemical Technology of Organic Dyes and Phototropic Compounds
Russian Federation, Saint PetersburgVyacheslav P. Ganapolsky
Kirov Military Medical Academy
Email: ganvp@mail.ru
ORCID iD: 0000-0001-7685-5126
SPIN-code: 9872-8841
Colonel of the Medical Service, MD, Dr. Sci. (Medicine), Acting Head of the Pharmacology Department
Russian Federation, Saint PetersburgInessa V. Karpova
Institute of Experimental Medicine
Email: inessa.karpova@gmail.com
ORCID iD: 0000-0001-8725-8095
SPIN-code: 9874-4082
Dr. Sci. (Biology), Associate Professor, Senior Researcher of the Department of Neuropharmacology named after S.V. Anichkov
Russian Federation, Saint PetersburgReferences
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