Detection of Ebola Virus VP40 Protein using a Nanowire SOI Biosensor
- Авторы: Generalov V.M.1, Naumova O.V.2, Fomin B.I.2, P’yankov S.A.1, Khlistun I.V.1, Safatov A.S.1, Zaitsev B.N.1, Zaitseva E.G.2, Aseev A.L.2
-
Учреждения:
- Federal Service for the Surveillance in Consumer Rights and Human Well-Being
- Rzhanov Institute of Semiconductor Physics, Siberian Branch
- Выпуск: Том 55, № 6 (2019)
- Страницы: 618-622
- Раздел: Optical Information Technologies
- URL: https://journals.rcsi.science/8756-6990/article/view/212934
- DOI: https://doi.org/10.3103/S875669901906013X
- ID: 212934
Цитировать
Аннотация
Nanowire SOI biosensors are used to detect the Ebola virus VP40 protein by identifying its immune complexes with specific monoclonal antibodies (MCAs). It is shown that the reaction of specific interaction between VP40 and MCAs causes a more pronounced biosensor response than direct adsorption of VP40 or antibodies to their surface. The formation of antigen — antibody complexes leads to the accumulation of a negative charge on the biosensor surface. The protein and protein — antibody complexes are detected in real time (∼200–300 s per test sample).
Ключевые слова
Об авторах
V. Generalov
Federal Service for the Surveillance in Consumer Rights and Human Well-Being
Автор, ответственный за переписку.
Email: general@vector.nsc.ru
Россия, Kol’tsovo, Novosibirsk Region, 630559
O. Naumova
Rzhanov Institute of Semiconductor Physics, Siberian Branch
Email: general@vector.nsc.ru
Россия, pr. Akademika Lavrentyeva 13, Novosibirsk, 630090
B. Fomin
Rzhanov Institute of Semiconductor Physics, Siberian Branch
Email: general@vector.nsc.ru
Россия, pr. Akademika Lavrentyeva 13, Novosibirsk, 630090
S. P’yankov
Federal Service for the Surveillance in Consumer Rights and Human Well-Being
Email: general@vector.nsc.ru
Россия, Kol’tsovo, Novosibirsk Region, 630559
I. Khlistun
Federal Service for the Surveillance in Consumer Rights and Human Well-Being
Email: general@vector.nsc.ru
Россия, Kol’tsovo, Novosibirsk Region, 630559
A. Safatov
Federal Service for the Surveillance in Consumer Rights and Human Well-Being
Email: general@vector.nsc.ru
Россия, Kol’tsovo, Novosibirsk Region, 630559
B. Zaitsev
Federal Service for the Surveillance in Consumer Rights and Human Well-Being
Email: general@vector.nsc.ru
Россия, Kol’tsovo, Novosibirsk Region, 630559
E. Zaitseva
Rzhanov Institute of Semiconductor Physics, Siberian Branch
Email: general@vector.nsc.ru
Россия, pr. Akademika Lavrentyeva 13, Novosibirsk, 630090
A. Aseev
Rzhanov Institute of Semiconductor Physics, Siberian Branch
Email: general@vector.nsc.ru
Россия, pr. Akademika Lavrentyeva 13, Novosibirsk, 630090
Дополнительные файлы
