Determination of the Orientation of Phospholipid Molecules in Planar Structures from Raman Spectra


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Abstract

A method is proposed for determining the orientation of phospholipid molecules in planar structures from spectra of non-polarized Raman scattering. The method is based on the sensitivity of the intensity of lines of Raman scattering from vibrations of CH2 groups to the orientation of phospholipid molecules. The validity of the method is illustrated on a planar sample of a saturated phospholipid prepared by drying from a solution. The principal component analysis is demonstrated to be a convenient tool for analyzing the spatial distribution of molecule orientations in the sample.

About the authors

K. A. Okotrub

Institute of Automation and Electrometry, Siberian Branch

Email: lab21@iae.nsk.su
Russian Federation, pr. Akademika Koptyuga 1, Novosibirsk, 630090

V. A. Zykova

Institute of Automation and Electrometry, Siberian Branch

Email: lab21@iae.nsk.su
Russian Federation, pr. Akademika Koptyuga 1, Novosibirsk, 630090

S. V. Adishchev

Institute of Automation and Electrometry, Siberian Branch

Email: lab21@iae.nsk.su
Russian Federation, pr. Akademika Koptyuga 1, Novosibirsk, 630090

N. V. Surovtsev

Institute of Automation and Electrometry, Siberian Branch; Novosibirsk State University

Author for correspondence.
Email: lab21@iae.nsk.su
Russian Federation, pr. Akademika Koptyuga 1, Novosibirsk, 630090; ul. Pirogova 2, Novosibirsk, 630090

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