Том 2, № 1 (2024)
- Жылы: 2024
- Мақалалар: 5
- URL: https://journals.rcsi.science/2949-6497/issue/view/23959
Бүкіл шығарылым
EDITORIA
The Application of Artificial Intelligence in Scientific Papers Writing
Аннотация
This editorial article describes the challenges faced by modern scientific journals in the context of the increasing capabilities and accessibility of artificial intelligence. Examples of disclaimers regarding authors’ use of artificial intelligence tools are provided.
Food metaengineering. 2024;2(1):7-10
7-10
ORIGINAL EMPIRICAL RESEARCH
The development of a modified packaging material with antioxidant properties and the study of its sanitary and hygienic characteristics
Аннотация
Introduction: The development and use of modified polyolefin films as packaging materials is a promising direction for the improvement of modern food packaging, particularly dairy products. By targeted modification, it is possible to create an "active package" that has a set of desired properties. For example, the packaging can contain substances with antimicrobial or antioxidant effects that are activated when the packaged product comes into contact with them.Purpose: This study aims to investigate the effect of adding calcium carbonate to a polyethylene film and antioxidant dihydroquercetin on its sanitary and hygiene properties.Materials and methods: The objects of research were samples of developed film filled with calcium carbonate (CaCO3) and dihydroquercetin (DKV), which were selected. The development and production of the materials was carried out on an experimental site using an SJ-28 laboratory extruder. Samples of film were produced using a pre-prepared super concentrate. Sanitary, chemical, and organoleptic tests were performed in accordance with TRTS 005/2011 “On Packaging Safety” and GOST 34174-2017, as well as instructions from the Ministry of Health, MI 880-71.Results: Using the methodology of combining polymer bases, mineral and organic fillers in a melt, it was possible to produce a modified film packaging material. An algorithm for the technology was proposed and modes for obtaining samples with different contents of injected substances were worked out. Organoleptic and sanitary-chemical tests of the obtained samples confirmed their sanitary and hygiene safety at selected filler concentrations and extrusion conditions.Conclusions: The data obtained on the production of filled modified films showed the technological suitability of the chosen method, mode, and component composition. Comprehensive organoleptic and chemical-sanitary tests have shown the absence of excessive odor and the lack of migration of low-molecular-weight substances and oxidation products in model media.
Food metaengineering. 2024;2(1):11-22
11-22
Study of the fractional composition and xylanolytic activity of proteins produced by bacteria isolated from lignocellulosic biomass
Аннотация
Introduction: In recent years, natural bioactive compounds have become a leading trend in the food and nutraceutical industries due to the diversity of their chemical structures and functions, as well as their positive effects on human health. The most studied and used bioactive compounds include prebiotics. Xylooligosaccharides, obtained by enzymatic hydrolysis of xylan, are of particular interest among prebiotics. Screening of microorganisms isolated from lignocellulosic raw materials for their ability to produce xylanolytic enzymes seems promising.Purpose: To assess the fractional composition and xylanolytic activity of proteins produced by bacteria isolated from lignocellulosic raw materials of the Kaliningrad region – white lupine seeds.Materials and Methods: Isolation of proteins from the fermentation medium and their fractionation were carried out using preparative HPLC. The molecular weight of protein fractions was determined by electrophoresis.Results: 4 protein fractions were isolated from the culture fluid of bacteria of the genus Bacillus and their molecular masses were established, ranging from 35.60 to 246.10 kDa. It was shown that fraction No. 1 contains the largest amount of proteins with molecular weights of 242.30 kDa, 83.70 kDa and 35.90 kDa. It was revealed that in fraction No. 2 proteins with a molecular weight of 51.50 kDa (content was 63.60%) and 42.70 kDa (content was 18.30%) predominated. It was established that fraction No. 3 contains a large number of proteins with different molecular weights, fraction No. 4 contains proteins with molecular weights from 61.80 to 69.30 kDa, while the share of proteins with molecular weights of 69.30 kDa and 61.80 kDa accounting for 42.10 and 41.10%, respectively. It was revealed that proteins with molecular weights from 35.60 to 41.00 kDa have xylanolytic activity at a level of 107.33 units/g.Conclusions: A scheme for the isolation of proteins with xylanolytic activity from the culture fluid of Bacillus megaterium bacteria isolated from seeds of white lupin (Lupinus albus) has been developed. The obtained enzymes can be used in the transformation of agro-industrial waste to produce xylooligosaccharides.
Food metaengineering. 2024;2(1):23-33
23-33
SCOPING REVIEW
Application of omics methods to the study of endophytic microorganisms: Scoping Review
Аннотация
Introduction: Grain crops form the basis of food security in many regions, including Kuzbass. However, they face the threat of decreasing yields due to biotic stressors. An effective solution might be the use of endophytic microorganisms. Their interaction with plants, however, has not been sufficiently studied yet. Omics technology provides an opportunity to investigate these relationships in depth, leading to increased grain productivity. Purpose and Research Questions: Generalization and systematization of knowledge regarding endophytic microbes obtained through omics techniques.Materials and methods: A search was conducted from December 4 to 18, 2023, in the databases of Scopus, ScienceDirect, and GoogleScholar using keywords such as endophytes, endosphere, RNA, transcriptome, genes, wheat, barley, and oats. No time limit was set for the search. Out of 239 relevant publications, 62 were selected based on inclusion criteria. The subject field review was conducted using the PRISMA-ScR protocol.Results: As a result of the search, 62 sources were found dedicated to the study of endophytic behavior using modern molecular genetic research methods. The analysis revealed the following trends in the utilization of endophyte microorganisms: the development of microbial bio-stimulators for agricultural crops and the use as agents for biological control of plant diseases. These trends are consistent with earlier reviews on this subject area, but in contrast to previous studies, there is a focus on realizing the genetic potential of microbiota and exploring the possibilities of modernizing microbial genetic information.Conclusions: It is necessary to expand the scope of molecular genetic methods in the study of endophytic microorganisms, as their use as biocontrol agents could become a promising strategy for sustainable development in the agro-industry. Special attention should be paid to the investigation of the relationship between these microbes and cereals, as there is currently insufficient information available in the scientific literature on this subject.
Food metaengineering. 2024;2(1):34-50
34-50
Cellular meat cultivation: Scoping Review
Аннотация
Introduction: In light of the increasing global demographic growth, the meat industry is facing challenges in ensuring a sufficient level of meat production to meet the increasing global demand. In this context, plant proteins and other alternative protein sources represent promising options for sustainable replacement of animal proteins. Special attention has recently been paid to the development of cultured meat.Purpose: a comprehensive analysis of the current state and prospects for the development of alternative protein sources, including vegetable proteins and cultured meat, in the context of global challenges related to the need to meet the increasing demand for meat products while reducing the negative impact on the environment and ensuring the sustainability of food systems.Materials and Methods: To write a review of the subject field, sources were selected in the databases Scopus, Web of Science, PubMed. Of the 187 keyword-relevant sources, 79 met the inclusion criteria. The review of the subject field was based on the PRISMA-ScR protocol.Results: The reconstruction of meat tissue is difficult due to its complex structure, which includes a variety of cells, extracellular matrix, proteins, nutrients and growth factors, which poses certain challenges to modern tissue engineering and 3D tissue culture. Cellular agriculture, which produces agricultural products from cell cultures, requires the addition of a nutrient medium to provide the necessary conditions for cell proliferation, differentiation and maturation. Various cell types, including stem and satellite cells, are used to create cultured meat, with the key aspect being the selection of cells with high reproductive capacity.Conclusions: Cultured meat has been a revolutionary technological advancement for the global food industry and has been seen as a potential answer to environmental, sustainability, public health and animal welfare concerns. The spread of cultured meat in the near future is limited only by the lack of available technologies, while widespread acceptance by potential consumers has almost been achieved.
Food metaengineering. 2024;2(1):51-67
51-67