SIMULATED MICROGRAVITY CHANGES THE NUMBER OF MECHANICALLY GATED AND MECHANOSENSITIVE ION CHANNELS GENES TRANSCRIPTS IN RAT VENTRICULAR CARDIOMYOCYTES

Andre G. Kamkin; Камкин А. Г.; Vitaliy E. Kalashnikov; Калашников В. Е.; Boris S. Shenkman; Шенкман Б. С.; Mitko I. Mladenov; Младенов М. И.; Pavel V. Sutyagin; Сутягин П. В.; Valentin I. Zolotarev; Золотарев В. И.; Alexandra D. Zolotareva; Золотарева А. Д.; Anastasia S. Rodina; Родина А. С.; Viktor E. Kazansky; Казанский В. Е.; Olga V. Kamkina; Камкина О. В.; Vadim M. Mitrokhin; Митрохин В. М.; Oleg I. Orlov; Орлов О. И.

doi:10.31857/S2686738923600383

SIMULATED MICROGRAVITY CHANGES THE NUMBER OF MECHANICALLY GATED AND MECHANOSENSITIVE ION CHANNELS GENES TRANSCRIPTS IN RAT VENTRICULAR CARDIOMYOCYTES

作者: Kamkin A.¹, Kalashnikov V.², Shenkman B.², Mladenov .¹, Sutyagin P.¹, Zolotarev V.¹, Zolotareva A.¹, Rodina .¹, Kazansky V.¹, Kamkina O.¹, Mitrokhin V.¹, Orlov O.²
隶属关系:
1. Pirogov Russian National Research Medical University
2. State Scientific Center of Russian Federation Institute of Biomedical Problems, Russian Academy of Sciences
期: 卷 512, 编号 1 (2023)
页面: 428-432
栏目: Articles
URL: https://journals.rcsi.science/2686-7389/article/view/140838
DOI: https://doi.org/10.31857/S2686738923600383
EDN: https://elibrary.ru/PABJTC
ID: 140838

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The mechanoelectrical feedback in the heart is based on the work of mechanically gated (MGCs) and mechanosensitive (MSCs) channels. Since microgravity alters the heart’s morphological and physiological properties, we hypothesized that the expression of both MGCs and MSCs would be affected. We employed RNA transcriptome sequencing to investigate changes in the gene transcript levels of MGCs and MSCs in isolated rat ventricular cardiomyocytes under control conditions and in a simulated microgravity environment. For the first time, our findings demonstrated that simulated microgravity induces alterations in the gene transcript levels of specific MGCs, such as TRPM7, TRPV2, TRPP1, TRPP2, Piezo1, TMEM63A, TMEM36B, and known MSCs, including K_2P2.1, K_2P3.1, Kir6.1, Kir6.2, Na_V1.5, Ca_V1.2, K_V7.1. However, other voltage-gated channels and channels lacking a voltage sensor remained unaffected. These findings suggest that the altered expression of MGCs and MSCs could lead to changes in the net currents across the membrane, ultimately impacting the heart’s function.

关键词

rats, heart, ventricles, cardiomyocytes, mechanically gated and mechano-sensitive ion channels, microgravity, channel genes transcripts, expression genes

参考

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SIMULATED MICROGRAVITY CHANGES THE NUMBER OF MECHANICALLY GATED AND MECHANOSENSITIVE ION CHANNELS GENES TRANSCRIPTS IN RAT VENTRICULAR CARDIOMYOCYTES

全文:

详细

关键词

作者简介

Andre Kamkin

Vitaliy Kalashnikov

Boris Shenkman

Mitko Mladenov

Pavel Sutyagin

Valentin Zolotarev

Alexandra Zolotareva

Anastasia Rodina

Viktor Kazansky

Olga Kamkina

Vadim Mitrokhin

Oleg Orlov

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