THE EFFECT OF THE AGONIST CHOLECYSTOKININ-4 D-GB-115 ON THE CHARACTER OF MOTOR ACTIVITY OF  PARAMECIUM CAUDATUM

G. A. Gruzdev; Груздев Г. А.; L. V. Soboleva; Соболева Л. В.; A. A. Kamensky; Каменский А. А.

doi:10.31857/S2686738923600024

THE EFFECT OF THE AGONIST CHOLECYSTOKININ-4 D-GB-115 ON THE CHARACTER OF MOTOR ACTIVITY OF PARAMECIUM CAUDATUM

Autores: Gruzdev G.¹, Soboleva L.¹, Kamensky A.¹
Afiliações:
1. Lomonosov Moscow State University
Edição: Volume 510, Nº 1 (2023)
Páginas: 263-267
Seção: Articles
URL: https://journals.rcsi.science/2686-7389/article/view/135694
DOI: https://doi.org/10.31857/S2686738923600024
EDN: https://elibrary.ru/QHLRZY
ID: 135694

Citar

Texto integral

Acesso aberto
Acesso é fechado

Acesso está concedido
Acesso é fechado

Somente assinantes

Resumo
Sobre autores
Bibliografia
Arquivos suplementares
Estatísticas

Resumo

The study investigated the effect of GABA in various concentrations and D-GB-115 at a concentration of 10^–10 mol/ml on the behavior of Paramecium caudatum. It has been shown that GABA increases motor activity and changes the movement strategy of these protozoa, and the dose-effect relationship is domed, which can be explained by the presence of two types of GABA receptors in the outer membrane of paramecia: GABA-A and GABA-B. The range of active concentrations of GABA ranges from 10^–6 to 10^–16 mol/ml. The effect of pharmacological agents interacting with the GABA system on the behavior of infusoria: nembutal and D-GB-115.

Palavras-chave

paramecium caudatum, comparative physiology, cellular model, neurotransmitters

Bibliografia

Hobson-West P., Davies A. Societal Sentience: Constructions of the Public in Animal Research Policy and Practice // Sci Technol Human Values. 2018. V. 43. № 1. P. 671–693.
Gruzdev G.A., Karpukhina O.V., Yakunin V.G., et al. Effect of Low-Temperature Atmospheric Pressure Plasma on Paramecium caudatum Cell Culture // Moscow Univ Biol Sci Bull. 2022. V. 76. P. 244–248.
Gruzdev G.A., Karpukhina O.V., Inozemtsev A.N., et al. Method for Primary Screening of Pharmaceuticals on the Paramecium caudatum Eukaryotic Cell Model // Dokl Biochem Biophys. 2022. V. 502. P. 36–39.
Gudasheva T.A., Kir’Yanova E.P., Kolik L.G., et al. Design and synthesis of cholecystokinin-4 dipeptide analogues with anxiolytic and anxiogenic activities // Russ J Bioorganic Chem. 2007. V. 33. P. 383–389.
Schindelin J., Arganda-Carreras I., Frise E., et al. Fiji: An open-source platform for biological-image analysis // Nat. Methods. 2012. V. 9. P. 676–682.
Груздев Г.А., Карпухина О.В., Иноземцев А.Н., et al. Анализ двигательной активности инфузорий Paramecium caudatum как тест-система оценки свойств адреналина // Экспериментальная и клиническая фармакология V. 85. P. 41–45.
Schlaepfer C.H., Wessel R. Excitable Membranes and Action Potentials in Paramecia: An Analysis of the Electrophysiology of Ciliates // J Undergrad Neurosci Educ. 2015. V. 14. № 1. P. 82–6.
Ramoino P., Milanese M., Candiani S., et al. Gamma-amino butyric acid (GABA) release in the ciliated protozoon Paramecium occurs by neuronal-like exocytosis // J Exp Biol. 2010. V. 213. P. 1251–8.
Germann A.L., Burbridge A.B., Pierce S.R., et al. Activation of the Rat α1β2ε GABA-A Receptor by Orthosteric and Allosteric Agonists // Biomolecules. 2022. V. 12. P. 1–16.
Csaba G. The hormonal system of the unicellular Tetrahymena: a review with evolutionary aspects // Acta Microbiol Immunol Hung. 2012. V. 59. P. 131–156.
Siniscalchi A., Rodi D., Cavallini S., et al. Effects of cholecystokinin tetrapeptide (CCK4) and of anxiolytic drugs on GABA outflow from the cerebral cortex of freely moving rats // Neurochem Int. 2003. V. 42. P. 87–92.