Anti-tumor effect of high doses of carbon ions and x-rays during irradiation of Ehrlich ascites carcinoma cells ex vivo

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Abstract

The effect of carbon ions (12C) with the energy of 400 MeV/nucleon on the dynamics of induction and growth rate of solid tumors in mice under irradiation of Ehrlich’s ascites carcinoma cells (EAC) ex vivo at doses of 5–30 Gy relative to the action of equally effective doses of X-ray radiation was studied. The dynamics of tumor induction under the action of 12C and X-rays had a similar character and depended on the dose during 3 months of observation. The value of the latent period, both when irradiating cells with 12C and X-ray, increased with increasing dose, and the interval for tumor induction decreased. The rate of tumor growth after ex vivo irradiation of EAC cells was independent of either dose or type of radiation. The dose at which EAC tumors are not induced within 90 days was 30 Gy for carbon ions and 60 Gy for X-rays. The value of the relative biological effectiveness of carbon ions, calculated from an equally effective dose of 50% probability of tumors, was 2.59.

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About the authors

V. E. Balakin

P.N. Lebedev Physical Institute of the Russian Academy of Sciences

Author for correspondence.
Email: belyakovata@lebedev.ru

Corresponding Member, Branch “Physical-Technical Center” 

Russian Federation, Protvino

Т. А. Belyakova

P.N. Lebedev Physical Institute of the Russian Academy of Sciences

Email: belyakovata@lebedev.ru

Branch “Physical-Technical Center”

Russian Federation, Protvino

О. М. Rozanova

Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences

Email: belyakovata@lebedev.ru
Russian Federation, Pushchino

E. N. Smirnova

Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences

Email: belyakovata@lebedev.ru
Russian Federation, Pushchino

N. S. Strelnikova

P.N. Lebedev Physical Institute of the Russian Academy of Sciences

Email: belyakovata@lebedev.ru

Branch “Physical-Technical Center”

Russian Federation, Protvino

Е. А. Кузнецова

Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences

Email: belyakovata@lebedev.ru
Russian Federation, Pushchino

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Supplementary files

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2. Fig. 1. Dynamics of the appearance of tumors in mice after inoculation of ACE cells irradiated with: (a) carbon ions in the dose range of 5–30 Gy; (b) RI in doses of 20–60 Gy.

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3. Fig. 2. Dynamics of tumor growth in mice after inoculation of a suspension of ACE cells irradiated with 12C: (a) average relative tumor volumes depending on the days after tumor appearance; (b) The data in (a) are presented as the number of days for the tumor volume to increase 5 times the first measured volume. Volumes are normalized to the first measured volume ≥0.40 cm3. Statistical significance from the control group was assessed using the Mann–Whitney U test (* p ≤ 0.01).

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4. Fig. 3. Dynamics of tumor growth in mice after inoculation of a suspension of ACE cells irradiated with RR: (a) average relative tumor volumes depending on the days after tumor appearance; (b) The data in (a) are presented as the number of days for tumor volume to increase fivefold from the first volume measured. Volumes are normalized to the first measured volume ≥0.40 cm3. Statistical significance from the control group was assessed using the Mann–Whitney U test (* p ≤ 0.01).

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5. Fig. 4. Dependence of the number of mice without tumors on the dose of 12C and RI 90 days after inoculation of irradiated cells.

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