PEROXIDASE-LIKE ACTIVITY OF MAGNETIC NANOPARTICLES IN THE PRESENCE OF BLOOD PROTEINS

M. G. Gorobets; Горобец М. Г.; A. V. Bychkova; Бычкова А. В.; M. I. Abdullina; Абдуллина М. И.; M. V. Motyakin; Мотякин М. В.

doi:10.31857/S2686738923600322

PEROXIDASE-LIKE ACTIVITY OF MAGNETIC NANOPARTICLES IN THE PRESENCE OF BLOOD PROTEINS

Authors: Gorobets M.G.¹, Bychkova A.V.¹, Abdullina M.I.¹, Motyakin M.V.¹^,2
Affiliations:
1. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences
2. N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences
Issue: Vol 512, No 1 (2023)
Pages: 449-453
Section: Articles
URL: https://journals.rcsi.science/2686-7389/article/view/140849
DOI: https://doi.org/10.31857/S2686738923600322
EDN: https://elibrary.ru/OYKFPH
ID: 140849

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Abstract
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Abstract

The generation of hydroxyl radicals from hydrogen peroxide in aqueous solutions containing magnetic nanoparticles (MNPs), hemoglobin (Hb), immunoglobulin G (IgG), and human serum albumin (HSA) has been determined. The dependence of the rate of formation of the oxidized product of o-phenylenediamine (o-PDA) on the concentration of MNPs in solution, as well as on the concentration of proteins, was obtained. The peroxidase-like activity of MNPs was shown to decrease in the presence of HSA and IgG, while the addition of Hb to the reaction mixture was leading to its decrease and increase depending on protein concentration. The obtained effects can be used in the development of systems based on MNPs for theranostics, in particular, for suppression of tumor growth, and in predicting the ability of particles to catalyze the generation of reactive oxygen species (ROS) in vivo.

Keywords

Кeywords: reactive oxygen species, magnetic nanoparticles, ortho-phenylendiamine, immunoglobulin G, human serum albumin, hemoglobin, peroxidase-like activity, 2,3-diaminophenazine, hydroxyl radical

References

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