Email this article INVESTIGATION OF THE INTRACELLULAR DISTRIBUTION OF DOXORUBICIN IN MCF-7 HUMAN BREAST ADENOCARCINOMA CELLS BY THE METHOD OF CORRELATIVE SCANNING FLUORESCENT PROBE NANOTOMOGRAPHY
- Authors: Agapova O.I.1, Agapov I.I.1, Oleinikov V.A.2,3, Lyundup A.V.4, Yakovlev D.V.2, Markvicheva E.A.2, Gileva A.M.2, Solovyeva D.O.2, Mochalov K.E.2, Efimov A.E.1, Gautier S.V.1,5
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Affiliations:
- Shumakov National Medical Research Center of Transplantology and Artificial Organs
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
- Рeoples’ Friendship University of Russia (RUDN University)
- Sechenov University
- Issue: Vol 509, No 1 (2023)
- Pages: 119-123
- Section: Articles
- URL: https://journals.rcsi.science/2686-7389/article/view/135632
- DOI: https://doi.org/10.31857/S2686738923700178
- EDN: https://elibrary.ru/NBSPZM
- ID: 135632
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Abstract
The development of technologies for effective targeted drug delivery for oncotherapy requires the elaboration of new methods to analyze the features of micro- and nanoscale distributions of antitumor drugs in cells and tissues. This paper presents a new approach to three-dimensional analysis of intracellular distribution of cytostatics using fluorescence scanning optical-probe nanotomography technology. Correlative analysis of nanostructure and distribution of injected doxorubicin in studied MCF-7 human breast adenocarcinoma cells made it possible to reveal the features of drug penetration and accumulation in cells. The developed technology based on the principles of scanning optical probe nanotomography is applicable to study the distribution patterns of various fluorescent or fluorescence-labelled substances in cells and tissues.
About the authors
O. I. Agapova
Shumakov National Medical Research Center of Transplantology and Artificial Organs
Email: igor_agapov@mail.ru
Russian, Moscow
I. I. Agapov
Shumakov National Medical Research Center of Transplantology and Artificial Organs
Author for correspondence.
Email: igor_agapov@mail.ru
Russian, Moscow
V. A. Oleinikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
Email: igor_agapov@mail.ru
Russian, Moscow; Russian, Moscow
A. V. Lyundup
Рeoples’ Friendship University of Russia (RUDN University)
Email: igor_agapov@mail.ru
Russian, Moscow
D. V. Yakovlev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: igor_agapov@mail.ru
Russian, Moscow
E. A. Markvicheva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: igor_agapov@mail.ru
Russian, Moscow
A. M. Gileva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: igor_agapov@mail.ru
Russian, Moscow
D. O. Solovyeva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: igor_agapov@mail.ru
Russian, Moscow
K. E. Mochalov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: igor_agapov@mail.ru
Russian, Moscow
A. E. Efimov
Shumakov National Medical Research Center of Transplantology and Artificial Organs
Email: igor_agapov@mail.ru
Russian, Moscow
S. V. Gautier
Shumakov National Medical Research Center of Transplantology and Artificial Organs; Sechenov University
Email: igor_agapov@mail.ru
Russian, Moscow; Russian, Moscow
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