Study of the effect of UV irradiation of seed-bearing wheat on enzyme activity during germination
- Authors: Tupolskich T.I.1, Eroshenko A.A.1, Gucheva N.V.1, Doroshenko V.A.1, Gordeeva N.V.1, Fedorova A.V.1
-
Affiliations:
- Don State Technical University
- Issue: Vol 17, No 6-2 (2025)
- Pages: 687-697
- Section: Статьи
- Published: 30.12.2025
- URL: https://journals.rcsi.science/2658-6649/article/view/371785
- DOI: https://doi.org/10.12731/2658-6649-2025-17-6-2-1573
- EDN: https://elibrary.ru/JXNCJN
- ID: 371785
Cite item
Full Text
Abstract
Background. In this article, the study is devoted to the influence of UV irradiation on the activity of enzymes (amylase, catalase and peroxidase) in germinating seeds of winter wheat variety Rostovchanka 5. The aim of the study was to select the optimal modes of UV irradiation to stimulate germination. The results of the study indicate that short-term UV irradiation (3-5 minutes) can effectively stimulate enzyme activity in germinating winter wheat seeds, which can potentially increase germination and germination energy. These results may be useful for the development of innovative environmentally safe methods of pre-sowing seed stimulation. In this article, the study is devoted to the influence of UV irradiation on the activity of enzymes (amylase, catalase and peroxidase) in germinating seeds of winter wheat variety Rostovchanka 5. The aim of the study is to select the optimal modes of UV irradiation to stimulate germination.
Purpose. The aim of the study was to select the optimal modes of UV irradiation to stimulate germination
Materials and methods. In 2022-2024, research was conducted on wheat seeds of the Rostovchanka 5 variety bred in the Rostov region
Irradiation of seeds with UV rays after soaking in distilled water accelerates germination by 20%. The laboratory conducted experiments with repetition of 100 grains, moistening them every day with a tray of water in the thermostat.
Results. The results of the study indicate that short-term UV irradiation (3-5 minutes) can effectively stimulate enzyme activity in germinating winter wheat seeds, which can potentially increase germination and germination energy.
Conclusion. A mercury-quartz lamp for irradiation of winter soft wheat seeds was used for the study. The activity of enzymes (amylase, catalase, peroxidase) depending on irradiation time was determined.
The study showed that the use of UV irradiation to irradiate seeds improves their germination, activates biochemical processes and promotes plant growth. This demonstrates the importance of using UV irradiation in agriculture.
Keywords
About the authors
Tatiana I. Tupolskich
Don State Technical University
Author for correspondence.
Email: tupolskix@mail.ru
Head of the Department “Food Production Equipment and Technologies”
Russian Federation, 1, Gagarin Sq., Rostov-on-Don, 344000, Russian Federation
Arina A. Eroshenko
Don State Technical University
Email: ppipk19@mail.ru
Associate Professor of the Department “Food Production Equipment and Technologies”
Russian Federation, 1, Gagarin Sq., Rostov-on-Don, 344000, Russian Federation
Natalya V. Gucheva
Don State Technical University
Email: ngucheva@gmail.com
Senior Lecturer of the Department “Food Production Equipment and Technologies”
Russian Federation, 1, Gagarin Sq., Rostov-on-Don, 344000, Russian Federation
Valentina A. Doroshenko
Don State Technical University
Email: valy11164@mail.ru
Senior Lecturer of the Department “Food Production Equipment and Technologies”
Russian Federation, 1, Gagarin Sq., Rostov-on-Don, 344000, Russian Federation
Nadezhda V. Gordeeva
Don State Technical University
Email: nadinfomina@mail.ru
Senior Lecturer of the Department “Food Production Equipment and Technologies”, Deputy Director of the Department for Managing Educational Policy
Russian Federation, 1, Gagarin Sq., Rostov-on-Don, 344000, Russian Federation
Alla V. Fedorova
Don State Technical University
Email: afedorova@donstu.ru
Senior Lecturer of the Department “Engineering Geometry and Computer Graphics”
Russian Federation, 1, Gagarin Sq., Rostov-on-Don, 344000, Russian Federation
References
- Zhao, S., et al. (2014). Effects of ion beams pretreatment on damage of UV-B radiation on seedlings of winter wheat (Triticum aestivum L.). Applied Biochemistry and Biotechnology, 168, 2123–2135.
- Kondrateva, N., et al. (2020). Effect of treatment of seeds of grain crops by ultraviolet radiation before sowing. IOP Conference Series: Earth and Environmental Science, 433, 012039. https://doi.org/10.1088/1755-1315/433/1/012039
- Pournavab Foroughbakhch, et al. (2019). Ultraviolet radiation effect on seed germination and seedling growth of common species from Northeastern Mexico. Agronomy, 9, 269. https://doi.org/10.3390/agronomy9060269
- Layek, S., et al. (2022). Effect of gamma radiation on seed germination and seedling growth of snake gourd (Trichosanthes anguina L.). South African Journal of Botany, 145, 320–322. https://doi.org/10.1016/j.sajb.2021.07.039. EDN: https://elibrary.ru/UHLYSB
- Simonova, E., et al. (2020). The impact of UV irradiation of winter wheat seeds on enzymatic activity in the germination period. Indian Journal of Agricultural Research, 54, 232–236. https://doi.org/10.18805/IJARe.A-467. EDN: https://elibrary.ru/VHBBAN
- Ussenov, Y., et al. (2022). The effect of non-thermal atmospheric pressure plasma treatment of wheat seeds on germination parameters and α-amylase enzyme activity. IEEE Transactions on Plasma Science, 50, 330–340. https://doi.org/10.1109/TPS.2022.3145831. EDN: https://elibrary.ru/ZGFLUI
- Pelc, J., et al. (2020). Effect of fluoride on germination, early growth and antioxidant enzymes activity of three winter wheat (Triticum aestivum L.) cultivars. Applied Sciences, 10, 6971. https://doi.org/10.3390/app10196971. EDN: https://elibrary.ru/SPHOCH
- Putko, V., et al. (2024). Influence of magnetoplasma treatment on enzyme activity and germination of Triticum aestivum. Izvestiya vysshikh uchebnykh zavedeniy. The Volga region. Natural sciences, (1), 61–71. (In Russian). https://doi.org/10.21685/2307-9150-2024-1-6
- Lazim, S. (2023). The combined effect of seed priming with UV-C radiation and hydro-priming and hormonal priming by gibberellic acid on physiological parameters of wheat (Triticum aestivum L.). BioGecko, 12, 2078–2085.
- Kirova, E., et al. (2024). Exogenous cytokinin 4PU-30 modulates the response of wheat and einkorn seedlings to ultraviolet B radiation. Plants, 10, 1401. https://doi.org/10.3390/plants13101401. EDN: https://elibrary.ru/HIRATC
- Rudoy, D., et al. (2022). Methods for evaluating modern breeding materials for increasing yields. In: Genetic and radiation technologies in agriculture. Collection of reports of the I International Youth Conference (pp. 38–41). Obninsk. (In Russian).
- Benincasa, P., et al. (2020). Phenolic content and antioxidant activity of einkorn and emmer sprouts and wheatgrass obtained under different radiation wavelengths. Annals of Agricultural Sciences, 65, 68–76. https://doi.org/10.1016/j.aoas.2020.02.001. EDN: https://elibrary.ru/GZRLAI
- Hasanuzzaman, M., et al. (2020). Regulation of ROS metabolism in plants under environmental stress: A review of recent experimental evidence. International Journal of Molecular Sciences, 21, 8695. https://doi.org/10.3390/ijms21228695. EDN: https://elibrary.ru/IXWZMU
- Chen Dong, Z., et al. (2022). Plant responses to UV-B radiation: Signaling, acclimation and stress tolerance. Stress Biology, 2, 51. https://doi.org/10.1007/s44154-022-00076-9. EDN: https://elibrary.ru/LQQFSI
- Kosakivska, I. V., Vedenicheva, N. P., Babenko, L. M., Voytenko, L. V., Romanenko, K. O., & Vasyuk, V. A. (2022). Exogenous phytohormones in the regulation of growth and development of cereals under abiotic stresses. Molecular Biology Reports, 49(1), 617–628. https://doi.org/10.1007/s11033-021-06802-2. EDN: https://elibrary.ru/DKRQRG
- Zheng, Y., et al. (2023). Phytohormones regulate the abiotic stress: An overview of physiological, biochemical, and molecular responses in horticultural crops. Frontiers in Plant Science, 13, 1095363. https://doi.org/10.3389/fpls.2022.1095363. EDN: https://elibrary.ru/MMUNLZ
- Ellouzi, H., et al. (2023). Seed priming with salicylic acid alleviates salt stress toxicity in barley by suppressing ROS accumulation and improving antioxidant defense systems, compared to halo- and gibberellin priming. Antioxidants, 12, 1779. https://doi.org/10.3390/antiox12091779. EDN: https://elibrary.ru/QUKEYQ
- Li, Z., et al. (2017). The synergistic priming effect of exogenous salicylic acid and H2O2 on chilling tolerance enhancement during maize (Zea mays L.) seed germination. Frontiers in Plant Science, 8, 1153.
Supplementary files
