Polymorphism of microsatellite loci of cattle of the Limousine breed bred in the Republic of Bashkortostan

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Background. Modern molecular genetic technologies make it possible to monitor genetic resources, both at individual and population levels, and reduce the time of genetic improvement of herds.

Purpose. The given research aims to study the polymorphism of microsatellite loci in Limousine beef cattle bred in the Republic of Bashkortostan. The objectives include analysing the polymorphism of microsatellite loci in Limousine cattle, produced by absorptive crossing of Limousine bulls with dual-purpose cows (Simmental and Bestuzhev) and investigating the genetic structure and indicators of genetic diversity of Limousine cattle subpopulations.

Materials and methods. The research objects are young Limousine cattle of absorptive crossing grown in the limited liability farm "Miasnoi soyuz bashkirskikh proizvoditelei (Meat Union of Bashkir Producers) and SEC Yaroslavsky. The research was conducted in the genetic laboratories of the Bashkir State Pedagogical University named after M. Akmulla, the Federal State Budgetary Educational Institution of the Higher Education the Russian State Agricultural University named after K.A. Timiryazev and Bashkir Agricultural Research Institute of the Ufa Federal Research Centre of the Russian Academy of Sciences.

Results. The analysis of 16 STR loci in Limousine cattle DNA revealed 116 alleles in group I, where the maternal foundation was of the Simmental breed, and 74 alleles in group II, where the maternal foundation was of the Bestuzhev breed. The average number of alleles per locus was 7.25 in group I, and 4.63 in group II. The mean number of effective alleles per locus in groups I and II was 4.14 and 3.27, respectively. Group I exhibited high Shannon index values in the loci TGLA227, BM2113, TGLA53, CSSM66, and INRA023.  The observed and expected heterozygosity levels showed no significant differences. The spatial arrangement of genotypes in the principal coordinate system indicated that the studied subpopulations represent well-consolidated groups, with the preservation of individual genetic diversity in individuals, united by common ancestry and belonging to the same breed.

Conclusion. The obtained results can be used for the rational use of genetic resources of cattle of the Limousine breed and the development of genetically based breeding programs.

Sobre autores

Tatiana Sedykh

Ufa Branch of the Russian Academy of Sciences, Bashkir State Agrarian Institute; Bashkir State Pedagogical Institute named after M. Akmulla

Autor responsável pela correspondência
Email: nio_bsau@mail.ru
ORCID ID: 0000-0002-5401-3179
Rússia, 71, Oktyabrya Ave., Ufa, 450054, Russian Federation; 3a, Oktiabrskoi revolutsii Str., Ufa, 450008, Russian Federation

Diana Gareeva

Ufa Branch of the Russian Academy of Sciences, Bashkir State Agrarian Institute; Bashkir State Pedagogical Institute named after M. Akmulla

Email: diana.razgar@mail.ru
ORCID ID: 0009-0008-8279-6538
Rússia, 71, Oktyabrya Ave., Ufa, 450054, Russian Federation; 3a, Oktiabrskoi revolutsii Str., Ufa, 450008, Russian Federation

Niyaz Subkhankulov

Ufa Branch of the Russian Academy of Sciences, Bashkir State Agrarian Institute

Email: niyazsubhankulov@gmail.com
ORCID ID: 0000-0001-5038-2551
Rússia, 71, Oktyabrya Ave., Ufa, 450054, Russian Federation

Vladimir Kosilov

Orenburg State Agrarian University

Email: kosilov_vi@bk.ru
ORCID ID: 0000-0003-4754-1771
Rússia, 18, Cheliuskintsev Str., Orenburg, 460014, Russian Federation

Dmitry Zorin

Russian State Agrarian University – Moscow Agricultural Academy named after K.A. Timiryazev

Email: ethodmitry@mail.ru
ORCID ID: 0000-0002-5189-1355
Rússia, 49, Timiryazevskaya Str., Moscow, 127550, Russian Federation

Marianna Gladkikh

Russian State Agrarian University – Moscow Agricultural Academy named after K.A. Timiryazev

Email: marianna.gladkikh@rgau-msha.ru
ORCID ID: 0000-0002-2304-6058
Rússia, 49, Timiryazevskaya Str., Moscow, 127550, Russian Federation

Marina Selionova

Russian State Agrarian University – Moscow Agricultural Academy named after K.A. Timiryazev

Email: selionova@rgau-msha.ru
ORCID ID: 0000-0002-9501-8080
Rússia, 49, Timiryazevskaya Str., Moscow, 127550, Russian Federation

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