Molecular hydrogen in the technology of artificial insemination of cows

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Abstract

Background.  Cryopreservation of sperm from cattle is an effective method of breeding work. To increase the efficiency of cryopreservation, the improvement of synthetic diluents for freezing sperm is required. Special attention is paid to the search for new cryoprotectors capable of increasing sperm survival and preserving their fertility during cryopreservation.

Purpose. Evaluation of the effectiveness of artificial insemination of cows with sperm containing molecular hydrogen.

Materials and methods. For the experiment, 2 groups of animals were selected, 50 heads each. The cows of the control group were inseminated with sperm frozen in a BioXcell medium, and the animals of the experimental group with sperm frozen in a BioXcell medium with molecular hydrogen. Insemination of cows was carried out by a group method by synchronizing sexual hunting according to the "Ovsinh" scheme. The effectiveness of insemination was evaluated on the basis of rectal ultrasound examination data after insemination. A comparison was made of the effectiveness of artificial insemination of cows with sperm cryopreserved in an improved medium containing molecular hydrogen with the indicators of fertilization obtained using sperm frozen in an environment without molecular hydrogen.

Results. Fertilization of cows with sperm containing molecular hydrogen increased by 11.5% after 3 months compared with the use of cryopreserved sperm without molecular hydrogen.

Conclusion. The results of the experiments indicate that the use of molecular hydrogen in the composition of the medium for diluting the seed makes it possible to increase the fertilizing ability of bull sperm and increase the fertilization rates of cows during artificial insemination. 

About the authors

Marina N. Ivashchenko

Nizhny Novgorod State Agrotechnological University named after L.Y. Florentyev

Author for correspondence.
Email: kafedra2577@mail.ru
ORCID iD: 0000-0001-6642-8518
SPIN-code: 8510-8676

Cand. of Bio. Sc., Associate Professor

 

Russian Federation, 97, Gagarin Ave., Nizhny Novgorod, 603107, Russian Federation

Anna V. Deryugina

National Research Lobachevsky State University of Nizhny Novgorod

Email: deryugina@ibbm.unn.ru
ORCID iD: 0000-0001-8812-8559
SPIN-code: 7974-4600

Dr. of Bio. Sc., Associate Professor

 

Russian Federation, 23, Gagarin Ave., Nizhny Novgorod, 603022, Russian Federation

Alexey I. Erzutov

Nizhny Novgorod Enterprise LLC

Email: aerzutov@mail.ru
ORCID iD: 0009-0005-6175-4820
SPIN-code: 5401-2678

veterinarian

 

Russian Federation, 135, Sadovaya Str., Opytny Settlement, Kstovsky Municipal District, Nizhny Novgorod region, 607686, Russian Federation

Vladimir A. Petrov

Nizhny Novgorod State Agrotechnological University named after L.Y. Florentyev

Email: marina.31@rambler.ru
ORCID iD: 0000-0002-9190-0914
SPIN-code: 5979-1111

PhD student

 

Russian Federation, 97, Gagarin Ave., Nizhny Novgorod, 603107, Russian Federation

Andrey A. Belov

Nizhny Novgorod State Agrotechnological University named after L.Y. Florentyev

Email: andrey.raven@gmail.com
ORCID iD: 0000-0002-4869-5054
SPIN-code: 1394-7694

Cand. of Bio. Sc., Associate Professor

 

Russian Federation, 97, Gagarin Ave., Nizhny Novgorod, 603107, Russian Federation

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