Analysis of gene expression changes in canola in response to inoculation with biotic and abiotic elicitors to Alternaria brassicae

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Background. Alternaria brassicae is one of the most destructive pathogens of oilseed rape growth and production. Oilseed rape roots treated with biotic and abiotic elicitors have shown resistance to A. brassicae as a result of induced systemic resistance (ISR). In this experiment, we aimed to identify resistance genes in two rapeseed genotypes (Raphanus brassica and Brassica napus).

Methods. Pseudomonas fluorescens PF83 as a biotic elicitor and with Salicylic acid (SA) as an abiotic elicitors were used induced systemic resistance in rapeseed. The expression levels of six defense-related genes (VSP2, MYC2, PR-2, PR-3, PR-4 and PR-5) by Quantitative real-time PCR (qRT-PCR) at three times 24, 48 and 120 hpi.

Results. VSP2, MYC2, PR-3 and PR-4 gene expression levels increased in infected leaves by A. brassicae and in oilseed genotypes treated by PF83, while the expression levels of PR-2 and PR-5 were increased in leaves treated with SA. The results indicate that the induction of resistance genes depends on the type of elicitor, the biotic and abiotic elicitors interferes with phytohormones SA- and JA-pathways to A. brassicae, through the increase in the transcription of resistance-related genes. The abiotic elicitor is key to inducing resistance genes associated with SA-pathways, while the biotic elicitor is key to inducing resistance genes associated with JA-pathways. The results of our study provide a new understanding of the control mechanisms of A. brassica.

Conclusion. This study provides a new understanding of the control mechanisms of A. brassica. The abiotic elicitor is key to inducing resistance genes associated with SA-pathways, while the biotic elicitor is key to inducing resistance genes associated with JA-pathways.  This study explained, that the RR genome is responsible for disease resistance in oilseed R. brassica (AARR) to infection processes of A. brassicae, so we recommend that using R. brassica as resistance genotype to produce oilseed.

About the authors

Jawadayn Talib Alkooranee

Plant Department, College of Agriculture, Wasit University

Author for correspondence.
Email: jalkooranee@uowasit.edu.iq
ORCID iD: 0000-0003-2997-4637
Scopus Author ID: 56951124900
Iraq, Wasit, Iraq

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