The results of the study of the effects of glyphosate and antibiotics on the composition and functions of the intestinal microbiome of broilers

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Abstract

Background. The combined effect of residual amounts of pesticides and antibiotics in poultry feed can lead to a negative effect on the composition of the intestinal microbiome of livestock.

Purpose. To analyze changes in the composition of broilers intestinal cecum microbiome under the influence of glyphosate in an amount of 1 MPC for food products in isolation and with a combination of glyphosate with antibiotics and an anticoccidial drug.

Materials and methods. The experimental birds were divided into 4 groups: Group I - control, which received the basic diet (BD), experimental group II - BD with the addition of glyphosate; III experimental - BD with the addition of glyphosate and veterinary antibiotics; IV experimental - BD with the addition of glyphosate and anticoccidial drug. The composition of bacteria was determined by NGS sequencing on a MiSeq automatic sequencer (Illumina, Inc., USA). PICRUSt2 (v.2.3.0) software (https://github.com/picrust/picrust2) was used to perform functional activity prediction of the metagenome, gene families, and proteins.

 Results. The results showed that under the influence of glyphosate (experimental group II), on the 7th and 40th days of life of broilers, microorganisms of the phylum Proteobacteria were completely eliminated from the community of microorganisms in the chyme of the intestinal cecum; on the 14th day, their content decreased by 3 .7 times compared to control group I (P≤0.05). In groups III and IV, their number increased compared to group II to 3.1 and 7.9 times, respectively (P≤0.05). At 7 days of age in experimental group II, as well as at 7-40 days of age in experimental group III, the number of Ruminococcaceae and Oscillospiraceae bacteria decreased to 10.3 and 11.8%, respectively, compared to control group I (P≤0. 05). Changes in the composition of microbial taxa under the influence of pesticides and medicinal substances introduced into feed led to changes in 185 potential functional pathways (P≤0.05). Thus, the activity of the pathways of energy, carbohydrate, protein metabolism, fat metabolism, biosynthesis of coenzymes and cofactors, vitamins decreased in experimental groups II-IV compared to control group I (P≤0.05).

Conclusion. Our data indicate that during the experimental contamination of feed with the herbicide glyphosate, the introduction of veterinary antibiotics and anticoccidial drug into the feed in the caeca of the intestines of broiler chickens, changes in the structure of the microbiome occurred already at high taxonomic levels, and critical inhibition was also observed important potentially underlying functional pathways. This can negatively affect the host's body.

About the authors

Daria G. Tyurina

BIOTROF Limited Liability Company

Author for correspondence.
Email: tiurina@biotrof.ru
ORCID iD: 0000-0001-9001-2432
SPIN-code: 9917-5118
Scopus Author ID: 57219196023

Candidate of Economic Sciences, Deputy Director for Finance, Chief Biotechnologist of the Molecular Genetic Laboratory

 

Russian Federation, 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation

Elena A. Yildirim

St. Petersburg State Agrarian University; BIOTROF Limited Liability Company

Email: deniz@biotrof.ru
ORCID iD: 0000-0002-5846-5105
SPIN-code: 4479-7509
Scopus Author ID: 57059948100
ResearcherId: C-3770-2014

Doctor of Biological Sciences, Professor of the Department of Large Livestock, Chief Biotechnologist of the Molecular Genetic Laboratory

 

Russian Federation, building 2, Peterburgskoe highway, St. Petersburg, Pushkin, 196601, Russian Federation; 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation

Georgy Y. Laptev

St. Petersburg State Agrarian University; BIOTROF Limited Liability Company

Email: georg-laptev@rambler.ru
ORCID iD: 0000-0002-8795-6659
SPIN-code: 3600-5295
Scopus Author ID: 54414368800
ResearcherId: A-9395-2019

Doctor of Biological Sciences, Professor of the Department of Large Livestock, director

 

 

Russian Federation, building 2, Peterburgskoe highway, St. Petersburg, Pushkin, 196601, Russian Federation; 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation

Natalia I. Novikova

BIOTROF Limited Liability Company 

Email: novikova@biotrof.ru

Candidate of Biological Sciences, First Deputy Director

 

Russian Federation, 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation 

Larisa A. Ilyina

St. Petersburg State Agrarian University; BIOTROF Limited Liability Company

Email: ilina@biotrof.ru
ORCID iD: 0000-0003-2789-4844
SPIN-code: 5826-7525
Scopus Author ID: 57060452100
ResearcherId: C-3772-2014

Doctor of Biological Sciences, Professor of the Department of Large Livestock, Chief of the Molecular Genetics Laboratory

 

Russian Federation, building 2, Peterburgskoe highway, St. Petersburg, Pushkin, 196601, Russian Federation; 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation

Valentina A. Filippova

St. Petersburg State Agrarian University; BIOTROF Limited Liability Company

Email: filippova@biotrof.ru
ORCID iD: 0000-0001-8789-9837
SPIN-code: 4398-5340
Scopus Author ID: 57060101800
ResearcherId: AAE-2402-2022

Senior Lecturer at the Department of Large Livestock, Senior Biotechnologist at the Molecular Genetics Laboratory

 

Russian Federation, building 2, Peterburgskoe highway, St. Petersburg, Pushkin, 196601, Russian Federation; 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation

Andrey V. Dubrovin

BIOTROF Limited Liability Company 

Email: dubrovin@biotrof.ru

Candidate of Veterinary Sciences, Biotechnologist of the Molecular Genetic Laboratory

 

Russian Federation, 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation 

Kseniya A. Kalitkina

St. Petersburg State Agrarian University; BIOTROF Limited Liability Company

Email: kalitkina.xeniya@gmail.com
ORCID iD: 0000-0002-9541-6839
SPIN-code: 7893-2670
Scopus Author ID: 57280455100
ResearcherId: ADD-4706-2022

Assistant of the Department of Large Livestock, Biotechnologist of the Molecular Genetic Laboratory

 

Russian Federation, building 2, Peterburgskoe highway, St. Petersburg, Pushkin, 196601, Russian Federation; 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation

Ekaterina S. Ponomareva

BIOTROF Limited Liability Company

Email: kate@biotrof.ru
ORCID iD: 0000-0002-4336-8273
SPIN-code: 4260-6755
Scopus Author ID: 57262828600
ResearcherId: AGB-6728-2022

Biotechnologist of the Molecular Genetic Laboratory

 

Russian Federation, 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation

Irina A. Klyuchnikova

St. Petersburg State Agrarian University; BIOTROF Limited Liability Company

Email: klyuchnikova.irinaa@yandex.ru
ORCID iD: 0009-0008-6484-1235
SPIN-code: 8822-5738

master's student, Biotechnologist of the Molecular Genetic Laboratory

 

Russian Federation, building 2, Peterburgskoe highway, St. Petersburg, Pushkin, 196601, Russian Federation; 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation

Vasiliy A. Zaikin

BIOTROF Limited Liability Company

Email: dfcx@biotrof.ru
ORCID iD: 0009-0006-8029-9955

Biotechnologist of the Molecular Genetic Laboratory

 

Russian Federation, 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation

Elena P. Gorfunkel

BIOTROF Limited Liability Company

Email: elena@biotrof.ru
ORCID iD: 0000-0002-6843-8733
SPIN-code: 2958-6204

Quality controller

 

Russian Federation, 8, Malinovskaya Str., St. Petersburg, Pushkin, 196602, Russian Federation

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Согласие на обработку персональных данных с помощью сервиса «Яндекс.Метрика»

1. Я (далее – «Пользователь» или «Субъект персональных данных»), осуществляя использование сайта https://journals.rcsi.science/ (далее – «Сайт»), подтверждая свою полную дееспособность даю согласие на обработку персональных данных с использованием средств автоматизации Оператору - федеральному государственному бюджетному учреждению «Российский центр научной информации» (РЦНИ), далее – «Оператор», расположенному по адресу: 119991, г. Москва, Ленинский просп., д.32А, со следующими условиями.

2. Категории обрабатываемых данных: файлы «cookies» (куки-файлы). Файлы «cookie» – это небольшой текстовый файл, который веб-сервер может хранить в браузере Пользователя. Данные файлы веб-сервер загружает на устройство Пользователя при посещении им Сайта. При каждом следующем посещении Пользователем Сайта «cookie» файлы отправляются на Сайт Оператора. Данные файлы позволяют Сайту распознавать устройство Пользователя. Содержимое такого файла может как относиться, так и не относиться к персональным данным, в зависимости от того, содержит ли такой файл персональные данные или содержит обезличенные технические данные.

3. Цель обработки персональных данных: анализ пользовательской активности с помощью сервиса «Яндекс.Метрика».

4. Категории субъектов персональных данных: все Пользователи Сайта, которые дали согласие на обработку файлов «cookie».

5. Способы обработки: сбор, запись, систематизация, накопление, хранение, уточнение (обновление, изменение), извлечение, использование, передача (доступ, предоставление), блокирование, удаление, уничтожение персональных данных.

6. Срок обработки и хранения: до получения от Субъекта персональных данных требования о прекращении обработки/отзыва согласия.

7. Способ отзыва: заявление об отзыве в письменном виде путём его направления на адрес электронной почты Оператора: info@rcsi.science или путем письменного обращения по юридическому адресу: 119991, г. Москва, Ленинский просп., д.32А

8. Субъект персональных данных вправе запретить своему оборудованию прием этих данных или ограничить прием этих данных. При отказе от получения таких данных или при ограничении приема данных некоторые функции Сайта могут работать некорректно. Субъект персональных данных обязуется сам настроить свое оборудование таким способом, чтобы оно обеспечивало адекватный его желаниям режим работы и уровень защиты данных файлов «cookie», Оператор не предоставляет технологических и правовых консультаций на темы подобного характера.

9. Порядок уничтожения персональных данных при достижении цели их обработки или при наступлении иных законных оснований определяется Оператором в соответствии с законодательством Российской Федерации.

10. Я согласен/согласна квалифицировать в качестве своей простой электронной подписи под настоящим Согласием и под Политикой обработки персональных данных выполнение мною следующего действия на сайте: https://journals.rcsi.science/ нажатие мною на интерфейсе с текстом: «Сайт использует сервис «Яндекс.Метрика» (который использует файлы «cookie») на элемент с текстом «Принять и продолжить».