Enviar artigo por via de e-mail Structural Features of the Nucleolus in the Mouse Germinal Vesicle Oocyte Revealed by AFM, SEM, and ToF-SIMS
- Autores: Astafiev A.A.1, Gulin A.A.1,2, Osychenko A.A.1, Solodina A.E.1, Syrchina M.S.1, Titov A.A.1, Shakhov A.M.1, Pogorelov A.G.3, Pogorelova V.N.3, Panait A.I.3, Nadtochenko V.A.1,2,4
-
Afiliações:
- Semenov Institute of Chemical Physics
- Department of Chemistry
- Institute of Theoretical and Experimental Biophysics
- Institute of Problems of Chemical Physics
- Edição: Volume 12, Nº 7-8 (2017)
- Páginas: 444-447
- Seção: Article
- URL: https://journals.rcsi.science/2635-1676/article/view/220130
- DOI: https://doi.org/10.1134/S1995078017040048
- ID: 220130
Citar
Acesso aberto
Acesso está concedido
Somente assinantes
Resumo
The structural organization of the biological material in the nucleoli (Nucleolus-Like Bodies (NLBs)) in mouse GV oocytes has been studied with atomic force microscopy (AFM), scanning electron microscopy (SEM), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Experimental samples are prepared by quick plunge freezing, followed by freeze-drying, embedding in Epon epoxy resin, and sectioning with a microtome. The biological material in the NLBs is found to be organized as a domain structure of densely packed granules with sizes from tenths of a micron to a micron.
Sobre autores
A. Astafiev
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Rússia, Moscow, 119991
A. Gulin
Semenov Institute of Chemical Physics; Department of Chemistry
Autor responsável pela correspondência
Email: aleksandr.gulin@phystech.edu
Rússia, Moscow, 119991; Moscow, 119991
A. Osychenko
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Rússia, Moscow, 119991
A. Solodina
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Rússia, Moscow, 119991
M. Syrchina
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Rússia, Moscow, 119991
A. Titov
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Rússia, Moscow, 119991
A. Shakhov
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Rússia, Moscow, 119991
A. Pogorelov
Institute of Theoretical and Experimental Biophysics
Email: aleksandr.gulin@phystech.edu
Rússia, Pushchino, Moscow oblast, 142290
V. Pogorelova
Institute of Theoretical and Experimental Biophysics
Email: aleksandr.gulin@phystech.edu
Rússia, Pushchino, Moscow oblast, 142290
A. Panait
Institute of Theoretical and Experimental Biophysics
Email: aleksandr.gulin@phystech.edu
Rússia, Pushchino, Moscow oblast, 142290
V. Nadtochenko
Semenov Institute of Chemical Physics; Department of Chemistry; Institute of Problems of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Rússia, Moscow, 119991; Moscow, 119991; Chernogolovka, Moscow oblast, 142432
Arquivos suplementares
