Structural Features of the Nucleolus in the Mouse Germinal Vesicle Oocyte Revealed by AFM, SEM, and ToF-SIMS


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The structural organization of the biological material in the nucleoli (Nucleolus-Like Bodies (NLBs)) in mouse GV oocytes has been studied with atomic force microscopy (AFM), scanning electron microscopy (SEM), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Experimental samples are prepared by quick plunge freezing, followed by freeze-drying, embedding in Epon epoxy resin, and sectioning with a microtome. The biological material in the NLBs is found to be organized as a domain structure of densely packed granules with sizes from tenths of a micron to a micron.

作者简介

A. Astafiev

Semenov Institute of Chemical Physics

Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Moscow, 119991

A. Gulin

Semenov Institute of Chemical Physics; Department of Chemistry

编辑信件的主要联系方式.
Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Moscow, 119991; Moscow, 119991

A. Osychenko

Semenov Institute of Chemical Physics

Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Moscow, 119991

A. Solodina

Semenov Institute of Chemical Physics

Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Moscow, 119991

M. Syrchina

Semenov Institute of Chemical Physics

Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Moscow, 119991

A. Titov

Semenov Institute of Chemical Physics

Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Moscow, 119991

A. Shakhov

Semenov Institute of Chemical Physics

Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Moscow, 119991

A. Pogorelov

Institute of Theoretical and Experimental Biophysics

Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Pushchino, Moscow oblast, 142290

V. Pogorelova

Institute of Theoretical and Experimental Biophysics

Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Pushchino, Moscow oblast, 142290

A. Panait

Institute of Theoretical and Experimental Biophysics

Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Pushchino, Moscow oblast, 142290

V. Nadtochenko

Semenov Institute of Chemical Physics; Department of Chemistry; Institute of Problems of Chemical Physics

Email: aleksandr.gulin@phystech.edu
俄罗斯联邦, Moscow, 119991; Moscow, 119991; Chernogolovka, Moscow oblast, 142432

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