Email this article Structural Features of the Nucleolus in the Mouse Germinal Vesicle Oocyte Revealed by AFM, SEM, and ToF-SIMS
- Authors: Astafiev A.A.1, Gulin A.A.1,2, Osychenko A.A.1, Solodina A.E.1, Syrchina M.S.1, Titov A.A.1, Shakhov A.M.1, Pogorelov A.G.3, Pogorelova V.N.3, Panait A.I.3, Nadtochenko V.A.1,2,4
-
Affiliations:
- Semenov Institute of Chemical Physics
- Department of Chemistry
- Institute of Theoretical and Experimental Biophysics
- Institute of Problems of Chemical Physics
- Issue: Vol 12, No 7-8 (2017)
- Pages: 444-447
- Section: Article
- URL: https://journals.rcsi.science/2635-1676/article/view/220130
- DOI: https://doi.org/10.1134/S1995078017040048
- ID: 220130
Cite item
Open Access
Access granted
Subscription Access
Abstract
The structural organization of the biological material in the nucleoli (Nucleolus-Like Bodies (NLBs)) in mouse GV oocytes has been studied with atomic force microscopy (AFM), scanning electron microscopy (SEM), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Experimental samples are prepared by quick plunge freezing, followed by freeze-drying, embedding in Epon epoxy resin, and sectioning with a microtome. The biological material in the NLBs is found to be organized as a domain structure of densely packed granules with sizes from tenths of a micron to a micron.
About the authors
A. A. Astafiev
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Russian Federation, Moscow, 119991
A. A. Gulin
Semenov Institute of Chemical Physics; Department of Chemistry
Author for correspondence.
Email: aleksandr.gulin@phystech.edu
Russian Federation, Moscow, 119991; Moscow, 119991
A. A. Osychenko
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Russian Federation, Moscow, 119991
A. E. Solodina
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Russian Federation, Moscow, 119991
M. S. Syrchina
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Russian Federation, Moscow, 119991
A. A. Titov
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Russian Federation, Moscow, 119991
A. M. Shakhov
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Russian Federation, Moscow, 119991
A. G. Pogorelov
Institute of Theoretical and Experimental Biophysics
Email: aleksandr.gulin@phystech.edu
Russian Federation, Pushchino, Moscow oblast, 142290
V. N. Pogorelova
Institute of Theoretical and Experimental Biophysics
Email: aleksandr.gulin@phystech.edu
Russian Federation, Pushchino, Moscow oblast, 142290
A. I. Panait
Institute of Theoretical and Experimental Biophysics
Email: aleksandr.gulin@phystech.edu
Russian Federation, Pushchino, Moscow oblast, 142290
V. A. Nadtochenko
Semenov Institute of Chemical Physics; Department of Chemistry; Institute of Problems of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Russian Federation, Moscow, 119991; Moscow, 119991; Chernogolovka, Moscow oblast, 142432
Supplementary files
