Constitutional constants as biomarkers of the state immune system of animal

Correlation constants are quantitative indicators of the relationship between the structural components of the immune system. A change in their magnitude and orientation is an indicator of the instability of the immune system, leading to the development of the disease. The purpose of this study was to determine the constitutional constants of immunological parameters during experimental BLV infection of sheep as biomarkers of immunogenesis. The level of immunoglobulins in blood serum was determined by simple radial immunodiffusion with poly- and monoclonal antibodies to IgM and Ig G. Determination of the relative number of neutrophils and lymphocytes in the blood of sheep was carried out according to standard methods. To assess the relationship between indicators, the Pearson correlation coefficient was used. The strength of correlation was assessed according to the Chaddock scale. At all stages of the study, a negative correlation between the number of neutrophils and lymphocytes remained, although its values were unstable. A decrease in the strength of the correlation between the parameters of neutrophils and lymphocytes was noted only on the 3rd day after infection, then on the 7th month of immunogenesis, which could be associated with an increase in the number of neutrophils and the development of bacterial complications. It was established that the constitutional constant of IgG/IgM changed from the 1st day of the immune response. A significant decrease in the strength of the correlation occurred in the period from 7 to 14 days of the immune response. The unstable of the dynamics of the relationship between “early” and “late” immunoglobulins shows how the pathogen blocks the synthesis of virus-neutralizing antibodies, which can lead to the development of immunopathology. But with a high negative correlation (r = –0.7–0.9) between the indicators of neutrophils and lymphocytes, the adaptive capabilities of the immune system are preserved, since it is this correlation constant that is a priority in viral infections. The conducted studies showed the promise of using constitutional constants as biomarkers of various stages of immunogenesis.