Email this article Correction of metabolic and structural disorders in bull spermatozoids during cryopreservation using molecular hydrogen
- Authors: Ivashchenko M.N.1,2, Deryuginа A.V.1, Belov A.A.1,2, Latushko М.I.3, Ignatiev P.S.3, Erzutov A.I.2, Lodyanоy M.S.2
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Affiliations:
- National Research Nizhny Novgorod State University named after N.I. Lobachevsky
- Nizhny Novgorod State Agrotechnological University
- Production Association “Ural Optical and Mechanical Plant named after E.S. Yalamov”
- Issue: No 2 (2024)
- Pages: 64-67
- Section: Zootechnics
- URL: https://journals.rcsi.science/2500-2082/article/view/255718
- DOI: https://doi.org/10.31857/S2500208224020137
- ID: 255718
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Abstract
The influence of molecular hydrogen on the fertility parameters and energy metabolism of spermatozoids of black-and-white Holstein bulls was studied. Sperm was diluted with sterile BioXcell medium (France), diluted in hydrogen water. We studied native sperm diluted with BioXcell and BioXcell with molecular hydrogen, as well as after deep freezing with and without pre-treatment with molecular hydrogen. The addition of molecular hydrogen to the medium for diluting sperm and subsequent freezing changed the functional status of cells after thawing, leading to an increase in their motility, an increase in the number of motile and fast sperm, and a decrease in the number of slow cells relative to the analyzed indicators of regenerative cells after cryopreservation that were not exposed to molecular hydrogen. Sperm motility after thawing was 12% higher (p ≤ 0.05), the average movement speed was 9% (p≤0.05), and the ATP content was two times higher than in sperm samples not treated with molecular hydrogen. The results of the experiment showed that the addition of molecular hydrogen to the semen extender improves the biological indicators of the quality of bull sperm, its fertility, and increases the energy metabolism of spermatozoids.
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About the authors
M. N. Ivashchenko
National Research Nizhny Novgorod State University named after N.I. Lobachevsky; Nizhny Novgorod State Agrotechnological University
Author for correspondence.
Email: kafedra2577@mail.ru
PhD in Biological Sciences
Russian Federation, Nizhny Novgorod; Nizhny NovgorodA. V. Deryuginа
National Research Nizhny Novgorod State University named after N.I. Lobachevsky
Email: kafedra2577@mail.ru
Grand PhD in Biological Sciences
Russian Federation, Nizhny NovgorodA. A. Belov
National Research Nizhny Novgorod State University named after N.I. Lobachevsky; Nizhny Novgorod State Agrotechnological University
Email: kafedra2577@mail.ru
PhD in Biological Sciences
Russian Federation, Nizhny Novgorod; Nizhny NovgorodМ. I. Latushko
Production Association “Ural Optical and Mechanical Plant named after E.S. Yalamov”
Email: kafedra2577@mail.ru
PhD in Engineering Sciences
Russian Federation, YekaterinburgP. S. Ignatiev
Production Association “Ural Optical and Mechanical Plant named after E.S. Yalamov”
Email: kafedra2577@mail.ru
PhD in Physical and Mathematical Sciences
Russian Federation, YekaterinburgA. I. Erzutov
Nizhny Novgorod State Agrotechnological University
Email: kafedra2577@mail.ru
PhD Student
Russian Federation, Nizhny NovgorodM. S. Lodyanоy
Nizhny Novgorod State Agrotechnological University
Email: kafedra2577@mail.ru
PhD in Biological Sciences
Russian Federation, Nizhny NovgorodReferences
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