Email this article Newly formated (tertiary) lymphoid structures in tumor growth
- Authors: Rustamkhanov R.A.1, Kzyrgalin S.R.1, Arybzhanov D.T.2, Gantsev K.S.1, Tursumetov D.S.1, Gantsev S.K.1
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Affiliations:
- Bashkir State Medical University
- South Kazakhstan Medical Academy
- Issue: Vol 7, No 4 (2022)
- Pages: 269-274
- Section: Oncology
- URL: https://journals.rcsi.science/2500-1388/article/view/111906
- DOI: https://doi.org/10.35693/2500-1388-2022-7-4-269-274
- ID: 111906
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Abstract
Aim – to reproduce tertiary lymphoid structures and study their features in a malignant neoplasm in the experiment.
Material and methods. Male white rats of the Wistar stress-resistant line were inoculated with the tumor strain sarcoma M1. Using the methods of microsurgical preparation, color lymphography and ultrasonic sonolipodestruction we isolated the lymph nodes without metastatic lesions (described as secondary in normal anatomy), the lymph nodes with metastases of M1 sarcoma, and the newly formed nodes (extrauterine or tertiary). Lymph nodes were examined macroscopically, micromorphometric studies were performed, histological and immunohistochemical signs of tertiary lymph nodes were determined. The obtained data were subjected to statistical analysis.
Results. In 60% of rats, in the right axillary region, in addition to the secondary lymph nodes described in normal anatomy, the tertiary lymph nodes were found on the side of the tumor. They were located near the histologically verified metastatic lymph node. The studied parameters of the main structural and functional zones of the tertiary lymph nodes had the following features: the enlarged marginal sinuses; a fewer number of lymphoid follicles; the average total area of blood vessels increased by 92% in relation to the metastatic lymph nodes and by 78% in relation to the secondary lymph nodes. The significant differences were registered with CD3, CD4, CD20, CD30 markers.
Conclusion. The study proved the possibility of reproducing tertiary lymphoid nodes in the experiment with malignant growth and revealed a number of their features.
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##article.viewOnOriginalSite##About the authors
Rasul A. Rustamkhanov
Bashkir State Medical University
Author for correspondence.
Email: weather86@mail.ru
ORCID iD: 0000-0002-0355-1399
assistant of the Department of Oncology with the courses on oncology and pathological anatomy
Russian Federation, UfaShamil R. Kzyrgalin
Bashkir State Medical University
Email: ufa.shamil@gmail.com
ORCID iD: 0000-0001-9721-108X
PhD, Associate professor of the Department of Oncology with the courses on oncology and pathological anatomy
Russian Federation, UfaDauranbek T. Arybzhanov
South Kazakhstan Medical Academy
Email: davran_a@mail.ru
ORCID iD: 0000-0002-0237-9064
PhD, Associate professor of the Department of Surgical Disciplines
Kazakhstan, ShymkentKamil Sh. Gantsev
Bashkir State Medical University
Email: gantseff@mail.ru
ORCID iD: 0000-0002-7562-5684
PhD, Professor of the Department of Oncology with the courses on oncology and pathological anatomy
Russian Federation, UfaDavlat S. Tursumetov
Bashkir State Medical University
Email: ufa.davlat@gmail.com
ORCID iD: 0000-0003-4069-6594
PhD, Associate professor of the Department of oncology with the courses on oncology and pathological anatomy
Russian Federation, UfaShamil Kh. Gantsev
Bashkir State Medical University
Email: prfg@mail.ru
ORCID iD: 0000-0003-2047-963X
PhD, Professor, Head of the Department of Oncology with the courses on oncology and pathological anatomy
Russian Federation, UfaReferences
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