Damage to neurons of the cerebral cortex in acute poisoning with baclofen and its combination with ethanol
- Authors: Romanova O.L.1,2, Golubev A.M.1,2, Churilov A.A.2, Sundukov D.V.1, Kislov M.A.3, Ershov A.V.3
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Affiliations:
- Peoples’ Friendship University of Russia
- Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
- The First Sechenov Moscow State Medical University (Sechenov University)
- Issue: Vol 8, No 4 (2022)
- Pages: 15-24
- Section: ORIGINAL STUDY ARTICLES
- URL: https://journals.rcsi.science/2411-8729/article/view/122456
- DOI: https://doi.org/10.17816/fm431
- ID: 122456
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Abstract
BACKGROUND: The number of poisoning with a muscle relaxant baclofen has increased recently. The brain is one of the target organs in baclofen poisoning.
AIM: To identify and quantify cerebral cortex neuron damage in baclofen and baclofen ethanol poisoning.
MATERIAL AND METHODS: A histological study of rat cerebral cortex was performed. The controls (n = 5) received neither baclofen nor ethanol. Groups 1 and 3 received baclofen (85 mg/kg), whereas groups 2 and 4 received baclofen (85 mg/kg) and ethanol (7 ml/kg). Groups 1 and 3 were euthanized 4 h after the drug administration, whereas groups 2 and 4 were euthanized after 24 h. Histological sections were stained with hematoxylin and eosin and by the Nissl method and examined by light microscopy (×400). The number of damaged neurons was calculated. Statistical processing was performed by the nonparametric Mann–Whitney method.
RESULTS: Neurons with reversible and irreversible changes in the controls accounted for 13% and 9%, respectively. At 4 h after baclofen administration, neurons with reversible and irreversible changes accounted for 22% and 21%; 4 h after baclofen and ethanol administration, 24% and 29%; 24 h after baclofen administration, 25% and 37%, respectively. Baclofen and ethanol administration caused an increase in the share of neurons with reversible (27%) and irreversible (41%) changes. The differences between group 3 and the control group were significant, and the difference was significant when group 4 was compared with the control group and group 3.
CONCLUSION: Understanding the processes in the brain during baclofen and baclofen ethanol administration will allow the provision of medical care to this category of patients more effectively. The signs of brain neuron damage, along with the results of forensic chemical analysis, can be used to establish the immediate cause of death.
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##article.viewOnOriginalSite##About the authors
Olga L. Romanova
Peoples’ Friendship University of Russia; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
Author for correspondence.
Email: olgpharm@yandex.ru
ORCID iD: 0000-0001-6356-9251
SPIN-code: 6727-9170
Cand. Sci. (Biol.)
Russian Federation, 8, Miklukho-Maklaya street, Moscow, 117198; MoscowArkady M. Golubev
Peoples’ Friendship University of Russia; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
Email: arkadygolubev@mail.ru
ORCID iD: 0000-0002-3165-0378
SPIN-code: 7890-2597
MD, Dr. Sci. (Med.)
Russian Federation, Moscow; MoscowAlexey A. Churilov
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
Email: sandro-knaz-him@mail.ru
ORCID iD: 0000-0003-1018-8257
SPIN-code: 8648-0654
Russian Federation, Moscow
Dmitriy V. Sundukov
Peoples’ Friendship University of Russia
Email: sundukov_dv@rudn.university
ORCID iD: 0000-0001-8173-8944
SPIN-code: 2968-7961
MD, Dr. Sci. (Med.)
Russian Federation, MoscowMaksim A. Kislov
The First Sechenov Moscow State Medical University (Sechenov University)
Email: smedik@gmail.com
ORCID iD: 0000-0002-9303-7640
SPIN-code: 3620-8930
MD, Dr. Sci. (Med.)
Russian Federation, MoscowAnton V. Ershov
The First Sechenov Moscow State Medical University (Sechenov University)
Email: salavatprof@mail.ru
ORCID iD: 0000-0001-5758-8552
SPIN-code: 2059-3248
MD, Dr. Sci. (Med.)
Russian Federation, MoscowReferences
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