Histomorphological changes in the lungs induced by poisoning with baclofen and its combination with ethanol (experimental study)

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Abstract

 Aim. Detection and assessment of histomorphological changes in the lungs in acute poisoning with Baclofen and its combination with ethanol three hours after poisoning. Material and methods. Experimental studies were conducted in 15 Wistar lineage rats divided into three groups. The control group included five intact animals, group 1 was treated with Baclofen at a dose of 85 mg/kg, group 2 — with a combination of Baclofen (85 mg/kg) and ethanol (7 mL/kg of 40% ethanol). Results. No pathological changes were observed in the lungs of the controls. Both treated groups manifested the capillary and venous plethora, sludge syndrome, atelectases and dystelectases, emphysema and infiltration of white blood cells into the interalveolar septa. Bronchioles in the group 2 animals contained secretion. Conclusion. In the conducted trials, a complex of pathological changes was observed in the lungs of animal models shortly after the administration of Baclofen, including circulatory disorders in all departments of the microcirculatory bed (plethora of capillaries and venules), emphysema, atelectases and dystelectases, leucocyte infiltration into the interalveolar septa. Morphometric studies are necessary to quantify the severity of histomorphological changes in the lungs.

About the authors

Olga L. Romanova

Peoples’ Friendship University of Russia; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology

Author for correspondence.
Email: olgpharm@yandex.ru
ORCID iD: 0000-0001-6356-9251

Cand. Sci. (Biol.), Assoc. Prof. of the Department of Forensic Medicine, Peoples’ Friendship University of Russia; Senior Researcher, V. A. Negovsky Scientific Research Institute of General Reanimatology of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology

Russian Federation, Moscow

Dmitriy V. Sundukov

Peoples’ Friendship University of Russia

Email: sudmed.rudn@yandex.ru
ORCID iD: 0000-0001-8173-8944

Dr. Sci. (Med.), Head of the Department of Forensic Medicine, Peoples’ Friendship University of Russia

Russian Federation, Moscow

Arkadiy M. Golubev

Peoples’ Friendship University of Russia; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology

Email: arkadygolubev@mail.ru

д.м.н., проф. кафедры судебной медицины ФГАОУ ВО РУДН; зав. лабораторией патологии клетки НИИ ОР ФНКЦ РР

Russian Federation, Moscow

Mikhail L. Blagonravov

Peoples’ Friendship University of Russia

Email: blagonravov_ml@rudn.ru
ORCID iD: 0000-0001-7838-0486

Dr. Sci. (Med.), Head of the Department of General Pathology and Pathological Physiology named after V. A. Frolov, Peoples’ Friendship University of Russia

Russian Federation, Moscow

Anton V. Ershov

Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology; I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: salavatprof@mail.ru
ORCID iD: 0000-0001-5758-8552

Dr. Sci. (Med.), Assoc. Prof., Leading Researcher, Laboratory of Experimental Studies, V. A. Negovsky Scientific Research Institute of General Reanimatology of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology; Prof., I. M. Sechenov First Moscow State Medical University

Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Lung of a rat in group 2 (Baclofen, 85 mg/kg). Emphysema. Thinning of the interalveolar septa in the emphysema zone.Magnification 400. Hematoxylin and eosin staining

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3. Fig. 2. Lung of a rat in group 2 (Baclofen, 85 mg/kg). Infiltration of interalveolar septa with leukocytes. Magnification 400. Hematoxylin and eosin staining

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4. Fig. 3. Lung of a rat in group 3 (Baclofen, 85 mg/kg + ethanol). Thickening of the interalveolar septa due to edema, infiltration of the interalveolar septa with leukocytes. Magnification 400. Hematoxylin and eosin staining

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5. Fig. 4. Lung of a rat in group 3 (baclofen, 85 mg/kg + ethanol). Secretion in the lumen of the bronchiole. Magnification 400. Hematoxylin and eosin staining

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6. Fig. 5. Lung of a rat in group 3 (baclofen, 85 mg/kg + ethanol). Thickening of intraalveolar septi due to edema. Infiltration of intraalveolar septi with WBC. Magnification 400. Hematoxylin and eosin staining

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Copyright (c) 2020 Romanova O.L., Sundukov D.V., Golubev A.M., Blagonravov M.L., Ershov A.V.

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