Comparative anatomical and histological research of the roots of Rheum Palmatum L. And Rheum Officinale B.

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Abstract

Introduction. The aim of the study is to perform the anatomical and histological analysis of the roots of Rheum palmatum L. and Rheum officinale B., and also to check histochemical reactions for the presence of anthracene derivatives.

Material and methods. The methodological basis of the anatomical and histological studies was the standard method for the preparation of micro-preparations of roots of Rheum palmatum L. and Rheum officinale B. described in the State Pharmacopoeia of the Russian Federation, XIV edition (OFS.1.5.3.003.15).

Results and discussion. Anatomical and histological studies of Rheum palmatum L. and Rheum officinale B. raw materials confirmed the roots of Rheum palmatum L. to have classical secondary structure, while the roots of Rheum officinale B. have classical primary structure. The histochemical reaction to anthracene derivatives with 10% alkali solution was carried out, it resulted in cherry-red staining of core rays and some structures of cortical parenchyma of the fragment of Rheum palmatum L., while the roots of Rheum officinale B. failed to stain.

Conclusions. The anatomical and histological studies of the rhizomes and roots of Rheum palmatum L. and Rheum officinale B. demonstrate the difference in the structure of closely related species of plants. It makes possible to identify the medicinal raw material of these plants.

About the authors

Ksenia N. Semenyuta

Orenburg State Medical University

Author for correspondence.
Email: evdkn@mail.ru

Assistant of the Department of Management and Economics of Pharmacy, Pharmaceutical Technology and Pharmacognosy

Russian Federation, Orenburg

Anna A. Shmygareva

Orenburg State Medical University

Email: a.shmygareva@mail.ru

Doctor of Pharmaceutical Sciences, Associate Professor, Professor of the Department of Management and Economics of Pharmacy, Pharmaceutical Technology and Pharmacognosy

Russian Federation, Orenburg

Anatoly N. Sankov

Orenburg State Medical University

Email: a.n.sankov@mail.ru

Candidate of Medical Sciences, Associate Professor, Head of the Department of Management and Economics of Pharmacy, Pharmaceutical Technology and Pharmacognosy

Russian Federation, Orenburg

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Supplementary files

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1. JATS XML
2. Fig. 1. A cross-section of the root of Rheum palmatum: a — (×100), b — (×400). Designations: 1 — tube; 2 — phelloderm; 3 — parenchyma of the cortex

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3. Fig. 2. A cross-section of the root of Rheum palmatum: a — (×100), b — (×400). Designations: 1 — core rays; 2 — xylem vessels; 3 — secondary xylem; 4 — cambium; 5 — druse; 6 — primary parenchyma

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4. Fig. 3. A cross-section of the root of Rheum palmatum: a — (×100), b — (×400). Designations: 1 — cambium; 2 — core beams; 3 — phelloderm; 4 — cork

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5. Fig. 4. Cross-section of root of Rheum officinale (×400). Designation: 1 — parenchyma of the primary cortex; 2 — exoderm; 3 — phloem; 4 — xylem; 5 — endoderm; 6 — parenchyma of the secondary cortex; 7 — stopper

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6. Fig. 5. A cross-section of the root of Rheum officinale: a — (×100); b — (×400). Designations: 1 — cork; 2 — phelloderm; 3 — parenchyma of the primary cortex; 4 — closed collateral bundle; 5 — endoderm; 6 — secondary xylem; 7 — parenchyma of the secondary cortex

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Copyright (c) 2020 Semenyuta K.N., Shmygareva A.A., Sankov A.N.

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