Experimental evaluation of the galvanophoretic dentin nanoimpregnation for recurrent caries prevention

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Abstract

Aim – to study the dentine galvanophoretic impregnation with nanoparticles of copper–calcium hydroxide (CCH) in the teeth with carious cavities.

Material and methods. The study was conducted on 21 teeth removed for various medical indications, with a concomitant diagnosis "dental caries". After mechanical treatment, the cavities were washed with saline solution, then CCH paste was placed on the cavity floor, covered with aluminum foil, drained and isolated with temporary fillings. To maintain the osmotic balance, the teeth were placed in a laboratory model: a plastic bath with saline solution and a melamine sponge, for a period of 1, 14 and 30 days. Then the longitudinal teeth sections were prepared for the electron microscopic examination.

Results. During the CCH galvanophoresis, the dentine impregnation with copper nanoparticles in the area of cavity floor occurred already during the first day of the procedure. The penetration depth reached 10.1 microns. The maximum depth of penetration of particles into the dentin (up to 30 microns) was detected after the 30-days-galvanophoresis. The maximum values of the diffusion coefficient and its velocity were recorded after the first day. With an increase in the time of galvanophoresis to 14 and 30 days, these indicators decreased.

Conclusion. The described method may be used as an option of choice for the prevention of recurrent dental caries, especially in the presence of deep cavities.

About the authors

Aleksandra R. Bessudnova

Tver State Medical University

Author for correspondence.
Email: bessudnova.aleksa@yandex.ru
ORCID iD: 0000-0001-9565-7133

a postgraduate student of the Department of Periodontology

Russian Federation, Tver

Vitalii A. Rumyantsev

Tver State Medical University

Email: rumyancev_v@tvgmu.ru
ORCID iD: 0000-0001-6045-3333

PhD, Professor, Head of the Department of Periodontology

Russian Federation, Tver

Georgii A. Frolov

National University of Science and Technology "MISIS"

Email: georgifroloff@yandex.ru
ORCID iD: 0000-0003-1460-6030

PhD, Associate professor of the Department of Physical Chemistry

Russian Federation, Moscow

Alisa V. Blinova

Tver State Medical University

Email: blinova-alisa@mail.ru
ORCID iD: 0000-0002-4315-163X

a postgraduate student of the Department of Periodontology

Russian Federation, Tver

Vladimir V. Bityukov

Tver State Medical University

Email: bitykova_l@mail.ru
ORCID iD: 0000-0003-0479-4971

assistant of the Department of Dentistry

Russian Federation, Tver

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Supplementary files

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2. Figure 1. Experimental laboratory model (A), tooth sections with carious cavities (B).

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3. Figure 2. Results of transmission electron microscopy (A) and X-ray fluorescence elemental analysis (B) of the preparation Cupral®.

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4. Figure 3. Electronic microphotographs of tooth sections after galvanophoresis procedures of the preparation Cupral® during A – 1 day, B – 14 days, C – 30 days.

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5. Figure 4. Concentration of copper-nanoparticles in the tooth dentine during galvanophoresis of Cupral® paste for different time periods: red curve – 1 day, yellow curve – 14 days, purple curve – 30 days.

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Copyright (c) 2023 Bessudnova A.R., Rumyantsev V.A., Frolov G.A., Blinova A.V., Bityukov V.V.

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