Current aspects of standardization of Spirulina platensis biomass

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Abstract

The food spirulina [Spirulina platensis (Nordst.) Geitl.] is a blue-green multicellular filamentous microscopic alga widely cultivated in many countries of the world, including the Russian Federation. The aim of this study is to develop approaches to the standardization of biomass of Spirulina platensis. As a result of the conducted research, approaches to the standardization of biomass of Spirulina platensis have been developed. It is aimed to determine the content of β-carotene, phycocyanin and proteins by using TLC and spectrophotometry. It was determined that the content of β-carotene in the samples of biomass of Spirulina platensis calculated on absolutely dry raw material varies from 63.0 mg% to 94.0 mg%, phycocyanin from 3.50% to 4.30% and protein from 53.50% to 61.09%. It was established that β-carotene, C-phycocyanin and protein were the most important diagnostically significant biologically active compounds of the biomass of Spirulina platensis. The analytical aspects of the study of Spirulina platensis biomass considered in this paper are the scientific basis for the substantiation of the creation of pharmaceuticals based on it.

About the authors

Anna A. Kosenko

Samara State Medical University

Author for correspondence.
Email: a.a.kosenko@samsmu.ru

Postgraduate student, Department of Pharmaceutical Technology with the Course of Biotechnologies

Russian Federation, Samara

Sergey V. Pervushkin

Samara State Medical University

Email: texnologi-samgmu@yandex.ru

Doctor of Pharmaceutical Sciences, Professor, Professor of the Department of Pharmaceutical Technology with the Course in Biotechnologies

Russian Federation, Samara

Nikolai N. Zhelonkin

Samara State Medical University

Email: texnologi-samgmu@yandex.ru

Candidate of Pharmaceutical Sciences, Associate Professor of the Department of Pharmaceutical Technology with the Course in Biotechnologies

Russian Federation, Samara

Anna V. Kurkina

Samara State Medical University

Email: a.v.kurkina@samsmu.ru

Doctor of Pharmaceutical Sciences, Head of the Department of Pharmaceutical Technologies with the Course of Biotechnologies

Russian Federation, Samara

References

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  5. Kurkin VA. Fundamentals of phytotherapy: textbook. Samara; 2009. (In Russ.)
  6. Parchevskaya DS, Drobetskaya IV, Minyuk GS. System characteristics of Spirulina platensis (nordst.) geitler under industrial conditions. Ecologiya morya. 2002;60:71–74. (In Russ.)
  7. Pervushkin SV, Voronin AV, Sokhina AA. Spirulina biomass: research and prospects of use. Samara; 2004. (In Russ.)
  8. Pervushkin SV, Markova II, Kurkin VA, Zhelonkin NN. The development of the methodics of the quantitative determination of content of β-carotene and phycocyanin in the biomass of spirulina platensis. Fundamentalnye issledovaniia. 2013;(8–6):1426–1429. (In Russ.)

Supplementary files

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2. Fig. 1. Electronic spectra of the hexane solution of the total carotenoids obtained from the biomass of Spirulina platensis (1) and the test solution after the purification on Al2O3 (2)

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3. Fig. 2. Electronic spectra of hexane solution of β-carotene

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4. Fig. 3. Electronic spectra of phycocyanin solution, obtained from Spirulina platensis biomass

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5. Fig. 4. Electronic spectrum of solutions of water extraction from Spirulina platensis biomass

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6. Fig. 5. Electronic spectrum of the test solution obtained with the use of phosphate buffer from Spirulina platensis biomass

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Copyright (c) 2021 Kosenko A.A., Pervushkin S.V., Zhelonkin N.N., Kurkina A.V.

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