The study of morphological and anatomical features of the herb astragalus glycyphyllus L.

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Abstract

Aim – to establish the morphological, anatomical and microdiagnostic features of the stem, leaf blade, calyx and corolla of Astragalus glycyphyllus in accordance with the regulatory documentation.

Material and methods. The object of the study was the herb Astragalus glycyphyllus harvested in the Kursk region in 2021 during the period of mass flowering of the plant. The State Pharmacopoeia XIV standard was used for macro- and microscopic analysis.

Results. The raw material of the licorice-leaved astragalus consists of non-woody leafy stems 20-25 cm long with flowers, whole and crushed leaves of a grayish-green color, parts of the stem and individual flowers of a greenish-yellow color. The microscopic analysis presented the stem of Astragalus glycyphyllus having a round-ribbed shape, covered on the outside with epidermis with straight-walled cells, with longitudinally wrinkled cuticle. Leaf cells have slightly sinuous walls with anomocytic stomata. The rachis in cross section has a triangular shape with small lateral outgrowths and anomocytic stomata. The epidermis of the rachis is pubescent with simple bicellular hairs with rough surface. Cells of the epidermis of the calyx from the prosenchymal form (in the tube of the calyx) to the parenchyma (in the pharynx, on the teeth of the calyx), anomocytic stomata. At the base of the corolla there are epidermal cells of a prosenchymal form, straight-walled with straight or beveled ends, in the limb of the corolla - epidermal cells of a prosenchymal form, straight-walled with a longitudinal wrinkled cuticle. The epidermis of the stem, leaves, rachis and calyx is pubescent with simple two-celled hairs on a short basal cell with a long terminal cell, with rough surface.

Conclusion. The macro- and microdiagnostic features of the herb Astragalus glycyphyllus were described to distinguish the raw material "Astragalus glycyphyllus herb".

About the authors

Tatyana A. Pozdnуakova

Orel State University named after I.S. Turgenev

Author for correspondence.
Email: pozdnyakova.tatyana.72@mail.ru
ORCID iD: 0000-0002-6058-6619

PhD, Associate professor, Department of Pharmacology, clinical pharmacology and pharmacy

Russian Federation, Orel

Roman A. Bubenchikov

Scientific and Production Association "Microgen"

Email: fg.ksmu@mail.ru
ORCID iD: 0000-0003-0955-6892

PhD, Associate professor, Head of the Department of Standardization and implementation

Russian Federation, Moscow

References

Supplementary files

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1. JATS XML
2. Figure 1. Microdiagnostic features of the stem of Astragalus glycyphyllus L. 1 – cross section of the stem of Astragalus glycyphyllus (40x), 2 – fragment of the stem cross section (100x), 3 – fragment of the parenchyma of the stem core with cells filled with starch grains (400x), 4 – fragment of the epidermis of the stem in the intercostal space with stomata and polygonal cells (200х), 5 – a fragment of the stem epidermis along the rib with a bicellular hair with rough surface (200х), 6 – a fragment of the stem epidermis with cells with longitudinal wrinkling of the cuticle.

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3. Figure 2. Microdiagnostic features of Astragalus glycyphyllus L. leaf. 1 – a fragment of the upper epidermis of a leaf with a rosette of epidermal cells at the site of attachment of the hair and epidermal cells with slightly sinuous walls (200x), 2 – a fragment of the upper epidermis of the leaf with a simple two-celled hair (100x), 3 – a fragment of the epidermis along the vein of the leaf with prosenchymal cells (200x), 4 – rosette of the epidermis at the site of hair attachment (100x), 5 – fragment of the leaf edge with simple bicellular hairs (100x), 6 – fragment of the lower leaf epidermis with stomata and sinuous epidermal cells (100x).

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4. Figure 3. Microdiagnostic features of rachis Astragalus glycyphyllus L. 1 – cross section of rachis (400x), 2 – fragment of rachis epidermis with a simple bicellular hair (100x), 3 – fragment of rachis epidermis with stoma (100x).

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5. Figure 4. Microdiagnostic features of flower Astragalus glycyphyllus L. 1 – a fragment of the calyx edge with simple bicellular pigmented and non-pigmented hairs (100x), 2 – a fragment of the epidermis of the fold of the corolla with epidermal cells with a longitudinal wrinkled cuticle (200x), 3 – a fragment of the epidermis at the base of the corolla with prosenchymal epidermal cells (200x).

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Copyright (c) 2022 Pozdnуakova T.A., Bubenchikov R.A.

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