INFRARED FOURIER SPECTROSCOPY OF BIOTIC DAMAGE TO SCOTS PINE NEEDLES

Global climate change, anthropogenic pollution, the development of epiphytotics and the mass reproduction of insect pests are the cause of the phytopathological state of tree species stands. To the understanding of the pathology mechanisms development of the tree’s structural elements (in particular, needles) will be promote analytical approach to the study of the tested plant organ. The study approach was implemented by us using the example of a comparative study of healthy and damaged Scots pine ( Pinus sylvestris L.) needles from suburban stands using infrared Fourier spectroscopy. The analysis of the IR spectra of healthy and damaged needles is based on a method for comparing the intensity ratios of absorption bands at characteristic frequencies (for bonds, functional groups, and the type of their oscillations). The assignment of absorption bands was carried out according to the literature data. In order to compare damaged samples of needles with the control, the absorption band intensity ratio was calculated for I 1375/2900, I 1695/1647, I 1506/898, I 1564/1550, I 1695/1533, I 1447/1369, I 1654/1730, I 1728/1477, I 2920/1600, I 3040/1600, I 3040/1508, I 1512/894 (subscripts indicate for which wave numbers the absorption intensity ratios were calculated). The analysis of the IR spectra needles array using this method showed what changes (qualitatively and quantitatively) at the level of molecular and structural parameters are characterized by shrinking and diseased needles compared with healthy ones.