Rooting of Vaccinium corymbosum L. microshoots cv. «Blue-Berry» in culture in vitro and ex vitro

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Abstract

This paper discusses a method of micropropagation of Vaccinium corymbosum L. cv. «Blue-Berry». The results showed that WPM supplemented with 1,0 mg/l of zeatin in combination with 0,1 mg/l of indolyl-3-butyric acid was more effective for the multiplication of blueberry axillary shoots. The maximum increase in the number of healthy axillary shoots was observed in the fourth subculture, whereas the phenomenon of hyperhydration (vitrification) began to appear in the fifth subculture. In addition, it was established that the presence of indolyl-3-butyric acid and 1,0 g/l of activated charcoal in the nutrient medium lead to the development of good root system of the Vaccinium corymbosum cv. «Blue-Berry». The highest mean number of roots formed per explant was obtained on WPM medium, supplemented with 0,5 mg/l indolyl-3-butyric acid after 10 weeks. Acclimatization of in vitro regenerated plantlets of Vaccinium corymbosum with a developed root system in ex-vitro conditions (pH 3,5–4) showed a 100% survival rate.

About the authors

Gamil Abuo El-Dis Mohamed

Kazan (Volga Region) Federal University

Email: gamil.rayan306@gmail.com

postgraduate student of Botany and Plant Physiology Department

Russian Federation, Kazan

Landysh Zavdetovna Khusnetdinova

Kazan (Volga Region) Federal University

Email: husnetdinova.l@mail.ru

candidate of biological sciences, associate professor of Botany and Plant Physiology Department

Russian Federation, Kazan

Olga Arnoldovna Timofeeva

Kazan (Volga Region) Federal University

Author for correspondence.
Email: olga.timofeeva@kpfu.ru

doctor of biological sciences, professor of Botany and Plant Physiology Department

Russian Federation, Kazan

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2. Figure 1 - Types of explants: A - stem segments; B - apical parts of the stem

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3. Picture 2 - Acclimatized plants-regenerants of high-growing blueberry variety Blue Berry: A - having a developed root system; B - not having a root system

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4. Figure 3 - Influence of BCI (0.5 mg / l) on rooting of blueberry microcuttings of Blue Berry variety

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5. Figure 4 - Rooted explants (%) V. corymbosum variety Blue Berry on WPM nutrient medium supplemented with various concentrations of auxin

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6. Figure 5 - Influence of different concentrations of auxin on rooting of blueberry microcuttings of Blue Berry cultivar after ten weeks

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7. Figure 6 - Adaptation of regenerant plants V. corymbosum variety Blue Berry

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8. Figure 7 - Influence of different concentrations of auxin on the viability of regenerated plants V. corymbosum cv. Blue Berry

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9. Figure 8 - Influence of different concentrations of BCI on the survival rate of regenerant plants V. corymbosum cv Blue Berry, which do not have a root system at the stage of acclimatization

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Copyright (c) 2018 Mohamed G.E., Khusnetdinova L.Z., Timofeeva O.A.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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