Supercritical extract from the Japanese sea brown algae Undaria pinnatifida as a source of bioactive compounds

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Abstract

Bioactive compounds contained in algae have practical applications in the pharmaceutical industry, production of human food and animal feed, and other fields. Since brown algae contain a wide range of bioactive substances, producing high-quality and chemically safe extracts on their basis is an urgent task. This study was aimed at characterization of supercritical extract from the corresponding algae, as well as the identification and evaluation of the content of bioactive compounds. The content of carotenoids, phenolic compounds and mannitol was determined by UV-Vis spectroscopy; a fatty acid analysis was carried out by GLPC; the qualitative composition of carotenoids and phenolic compounds was analyzed by HPLC. Supercritical CO2 extraction of the brown alga U. rinnatifida with EtOH as a polar modifier is characterized by high ejection efficiency of fatty acids, phenolic compounds and carotenoids. The latter profile of the collected supercritical extract consists of 14 representatives. In particular, fucoxanthin (58.1% of the sum), zeaxanthin (12.6% of the sum), and fucoxanthinol (14.5% of the sum) are the most abundant compounds. Palmitic acid, oleic acid, arachidonic acid, and eicosapentaenoic acids are the main of the 20 fatty acids found in the corresponding extract. However, the class of polyunsaturated fatty acids is predominant, which content of the ώ-6 and ώ-3 groups does not differ significantly. The total content of phenolic compounds is 13.45+0.43 mg/g of gallic acid equivalent. For instance, the most represented phenolic substances are epicatechin, epigallocatechin gallate, syringic acid, coumaric acid, ferulic acid and salicylic acid.

About the authors

O. V. Tabakaeva

Far Eastern Federal University

Email: yankovskaya68@mail.ru

A. V. Tabakaev

Far Eastern Federal University

Email: tabakaev92@mail.ru

References

  1. Craigie J.S. Seaweed extract stimuli in plant science and agriculture // Journal of Applied Phycology. 2011. Vol. 23, no. 3. P. 371–393. https://doi.org/10.1007/s10811-010-9560-4.
  2. Gerasimenko N.I., Martyyas E.A., Busarova N.G. Composition of lipids and biological activity of lipids and photosynthetic pigments from algae of the families Laminariaceae and Alariaceae // Chemistry of Natural Compounds. 2012. Vol. 48, no. 5. P. 737−741. https://doi.org/10.1007/s10600-012-0371-5. EDN: RGDLHZ.
  3. Суховеева М.В., Подкорытова А.В. Промысловые водоросли и травы морей Дальнего Востока: биология, распространение, запасы, технология переработки: монография. Владивосток: ТИНРО-центр, 2006. 243 с. EDN: QKYIZV.
  4. Табакаева О.В., Табакаев А.В. Биологически активные вещества потенциально промысловых бурых водорослей Дальневосточного региона // Вопросы питания. 2016. Т. 85. N 3. С. 126−133. EDN: WFGBEB.
  5. Rajauria G. In-vitro antioxidant properties of lipophilic antioxidant compounds from 3 brown seaweed // Antioxidants. 2019. Vol. 8, no. 12. P. 596−599. https://doi.org/10.3390/antiox8120596.
  6. Meresse S., Fodil M., Fleury F. Fucoxanthin, a marine-derived carotenoid from brown seaweeds and microalgae: a promising bioactive compound for cancer therapy // International Journal of Molecular Sciences. 2020. Vol. 21, no. 23. P. 9273−9278. https://doi.org/10.3390/ijms21239273.
  7. Jesumani V., Du H., Aslam M. Potential use of seaweed bioactive compounds in skincare // Marine Drugs. 2019. Vol. 17, no. 12. P. 688−694. https://doi.org/10.3390/md17120688.
  8. Gupta S., Abu-Ghannam N. Recent developments in the application of seaweeds or seaweed extracts as a means for enhancing the safety and quality attributes of foods // Innovative Food Science and Emerging Technologies. 2011. Vol. 12, no. 4. P. 600–609. https://doi.org/10.1016/j.ifset.2011.07.004.
  9. Salehi B., Sharifi-Rad J., Seca A.M.L., Pinto D.C.G.A., Michalak I., Trincone A., et al. Current trends on seaweeds: looking at chemical composition, phytopharmacology, and cosmetic applications // Molecules. 2019. Vol. 24, no. 22. P. 4182. https://doi.org/10.3390/molecules24224182.
  10. Herrero M., Mendiola J.A., Cifuentes A., Ibañez E. Supercritical fluid extraction: recent advances and applications // Journal of Chromatography A. 2010. Vol. 1217, no. 16. P. 2495–2511. https://doi.org/10.1016/j.chroma.2009.12.019.
  11. Herrero M., Cifuentes A., Ibañez E. Suband supercritical fluid extraction of functional ingredients from different natural sources: plants, food-by-products, algae and microalgae // Food Chemistry. 2006. Vol. 98, no. 1. P. 136−148. https://doi.org/10.1016/j.foodchem.2005.05.058.
  12. Valderrama J.O., Perrut M., Majewski W. Extraction of astaxantine and phycocyanine from microalgae with supercritical carbon dioxide // Journal of Chemical and Engineering. 2003. Vol. 48, no. 4. P. 827–830. https://doi.org/10.1021/je020128r.
  13. Razgonova M.P., Tekutyeva L.A., Podvolotskaya A.B., Zakharenko A.M., Golokhvast K. Zostera marina L.: supercritical CO2-extraction and mass spectrometric characterization of chemical constituents recovered from seagrass // Separations. 2022. Vol. 9, no. 7. P. 182. https://doi.org/10.3390/separations9070182.
  14. Razgonova M.P., Cherevach E.I., Tekutyeva L.A., Kirilenko N.S., Golokhvast K. Maackia amurensis Rupr. et Maxim.: supercritical CO2-extraction and mass spectrometric characterization of chemical constituents // Molecules. 2023. Vol. 28, no. 5. P. 2026. https://doi.org/10.3390/molecules28052026.
  15. Дизюров В.Д., Кулепанов В.Н., Шапошникова Т.В. Атлас массовых видов водорослей и морских трав российского Дальнего Востока: монография. Владивосток: ТИНРО-центр, 2008. 328 с. EDN: QKTKOP.
  16. Britton G., Liaaen-Jensen S., Pfander H. Carotenoids. Isolation and analysis. Basel: Birkhäuser Verlag, 1995. Vol. 1A. 328 p.
  17. Новак И.С. Количественный анализ методом газовой хроматографии. М.: Мир, 1978. 180 с.
  18. Carreau J.P., Dubacq J.P. Adaptation of a macro-scale method to the micro-scale for fatty acid methyl transesterification of biological lipid extracts // Journal of Chromatography A. 1978. Vol. 151. Р. 384−390. https://doi.org/10.1016/S0021-9673(00)88356-9.
  19. Табакаева О.В., Семилетова Е.В. Фитохимический состав потенциально промысловых бурых водорослей Дальневосточного региона // Химия природных соединений. 2015. N 4. С. 529−531.
  20. Pereira A.G., Otero P., Echave J., Carreira-Casais A., Chamorro F., Collazo N., et al. Xanthophylls from the sea: algae as source of bioactive carotenoids // Marine Drugs. 2021. Vol. 19, no. 4. P. 188. https://doi.org/10.3390/md19040188.
  21. Heffernan N., Smyth T., FitzGerald R.J., Vila-Soler A., Mendiola J., Ibáñez E., et al. Comparison of extraction methods for selected carotenoids from macroalgae and the assessment of their seasonal/spatial variation // Innovative Food Science & Emerging Technologies. 2016. Vol. 37. Р. 221–228. https://doi.org/10.1016/j.ifset.2016.06.004.

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