Effect of surfactants (sodium dodecyl sulfate, cetyltrimethylammonium bromide) on cell membrane permeability of red beet roots Beta vulgaris L.

The paper considers the effect of two surfactants - anionic sodium dodecyl sulfate (SDS) and cationic cetyltrimethylammonium bromide (CTAB) - on red beet root Beta vulgaris L. Damage to root tissues of Beta vulgaris L. was assessed in terms of an increased release of electrolytes and vacuolar pigments of betacyanins from cells using conductometric and spectrophotometric methods, respectively. It was shown that SDS and CTAB do not impair the cell membrane permeability at concentrations of up to 0.05 and 0.005 g/l, respectively. An increase in the concentration of these surfactants led to a subsequent rise in the electrolyte and betacyanin release from the beet tissues, indicating the negative effect of the surfactants. A good concentration dependence was observed, i.e., higher concentrations of the studied detergents correlated with higher values of the electric conductivity and optical density of the incubation solutions. A significant toxic effect was noted when the test plant was treated with the studied compounds at a concentration of 1 g/l. Thus, two hours after the onset of measurements, the electrical conductivity of the aqueous solution, in which the beet roots previously subjected to 30-min treatment with 1 g/l SDS and CTAB solutions were incubated, increased to 42 and 81 yS/cm, respectively. These values exceeded the reference values by 89 and 272%, respectively. At the same time, the betacyanin yield exceeded the reference values by 327 and 805%, respectively. The experiments showed that SDS and CTAB increase the permeability of plant cell membranes of both plasmalemma and tonoplast. The tested methods proved to be fast (three hours response time) and efficient. These methods can be used to rapidly assess the effect of surfactants on plant bodies, to study the membranotropic effect of substances, and to control the breeding crop plants in terms of their resistance to unfavourable conditions.

Beta vulgaris L., Beta vulgaris L., conductometric method, spectrophotometric method, electrolyte release, cell membrane permeability, surfactants