Full-genome sequencing of the Staphylococcus warneri strain isolated from oil-contaminated soil

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Abstract

Bacterial consortium bioremediation presents itself a promising approach to the treatment water, soil and the atmosphere for pollution by oil and its derivatives. In the territory of the Romashkinskoye oil field, Republic of Tatarstan, various decomposers were isolated from oil-contaminated black soil, including three that demonstrated oil resistance and ability to produce biosurfactants. The genome sequencing of the Staphylococcus warneri strain isolated in a consortium of decomposers was performed on the MiSeq Illumina plat-form. The average content of GC pairs in the genome comprised 32.7 %. Genome annotation was performed using the RAST server. The SEED viewer was applied for subsystem category distribution of predicted genes. The sequenced genome of Staphylococcus warneri strain was identified as containing 2535 protein coding sequences. The majority of annotated genes govern the synthesis of amino acids and their derivatives (255), carbohydrate (195) and protein metabolism (167), cofactors, vitamins, prosthetic groups and pigmented formations (87), nucleosides and nucleotides (78), fatty acid metabolism, lipids and isoprenoids (55), as well as DNA metabolism (68). The full-genome sequencing and genome annotation of the Staphylococcus warneri strain confirmed its hydrocarbon-oxidising properties. The yddN and yceB genes of uncharacterised proteins were identified as similar to alkanal monooxygenases likely to be involved in the biodegradation of alkanes. The three genes detected in this strain code the catechol-2,3-dioxygenase, fumarylacetoacetate hydrolase and salicylate-1-monooxygenase enzymes involved in the biodegradation of aromatic hydrocarbons. The obtained genome sequence data help to provide a better understanding of the process of hydrocarbon degradation (absorption) by the Staphylococcus warneri strain and its role in the bacterial consortium.

About the authors

I. A. Degtyareva

Tatar Research Institute of Agrochemistry and Soil Science, the “Kazan Scientific Centre of the Russian Academy of Sciences” Federal Research Centre; Kazan National Research Technological University

Email: peace-1963@mail.ru

E. V. Babynin

Kazan Federal University; Tatar Research Institute of Agrochemistry and Soil Science, the “Kazan Scientific Centre of the Russian Academy of Sciences” Federal Research Centre

Email: edward.b67@mail.ru

T. Yu. Motina

Tatar Research Institute of Agrochemistry and Soil Science, the “Kazan Scientific Centre of the Russian Academy of Sciences” Federal Research Centre

Email: motina.tatyana@mail.ru

M. I. Sultanov

Tatar Research Institute of Agrochemistry and Soil Science, the “Kazan Scientific Centre of the Russian Academy of Sciences” Federal Research Centre

Email: niiaxp2@mail.ru

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