A retrospective long-term assessment of antibody response in melioidosis animals
- Authors: Zakharova I.B.1, Tereshko D.L.1, Chirskov P.R.1, Bui T.2, Thomson S.3, Novitskaya I.V.1
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Affiliations:
- Volgograd Plague Control Research Institute, Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing
- Russian-Vietnamese Tropical Research and Technology Center
- Vietnam Bear Rescue Centre
- Issue: Vol 15, No 1 (2025)
- Pages: 173-177
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/2220-7619/article/view/292141
- DOI: https://doi.org/10.15789/2220-7619-ARL-17678
- ID: 292141
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Abstract
Melioidosis is an extremely fatal infection disease caused by the gram-negative bacterium Burkholderia pseudomallei. The causative agent of melioidosis is an environmental saprophyte and has significant adaptive potential allowing it to undergo rapid adaptation to a wide variety of ecological niches. The bacterium is a facultative intracellular pathogen that can infect many animal species as well as humans. B. pseudomallei has no pronounced tropism for certain tissues of the infected organism, which explains the diversity and non-specificity of clinical manifestations, the most common of which are pneumonia with or without bacteremia and sepsis. Тhis diagnosis is established on the basis of laboratory test data; cultured pathogen isolation is considered the gold standard. The indirect hemagglutination test remains a popular method for rapid melioidosis diagnostics, and increased paired serum antibody titer is an important criterion for making a diagnosis. The purpose of this work was to conduct a retrospective analysis of the long-term dynamic antibody response in Asiatic black bears that died from melioidosis and contact animals from the Vietnam Bear Rescue Center as well as molecular genetic analysis of isolated B. pseudomallei strains. Bacterial cultures were isolated from dead animals and identified by PCR as B. pseudomallei. Multilocus sequence typing showed that strains VP069 and VP044 had the same and hitherto undescribed sequence type (STnew), and strains VP161 and VP200 shared ST541. The pairwise strain identity with the same ST was confirmed by a core genome multilocus sequence typing. A retrospective analysis of 546 serum samples from 226 bears was carried out. All animals that died from melioidosis were seropositive for B. pseudomallei. Among the healthy bears studied, 24% were seropositive. It was found that in humans and bears there is a general trend in the dynamics of the antibody response. Similar to humans, bears with bacteriologically confirmed melioidosis were observed to have both higher and lower serum antibody titer, as well as a constant and fairly high titer, which shows the diagnostic ineffectiveness elevated paired serum antibody titer in melioidosis.
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##article.viewOnOriginalSite##About the authors
Irina B. Zakharova
Volgograd Plague Control Research Institute, Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing
Author for correspondence.
Email: zib279@gmail.com
DSc (Biology), Associate Professor, Leading Researcher, Laboratory of Pathogenic Burkholderia
Russian Federation, VolgogradD. L. Tereshko
Volgograd Plague Control Research Institute, Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing
Email: zib279@gmail.com
Researcher, Laboratory of Immunodiagnostic Preparation
Russian Federation, VolgogradP. R. Chirskov
Volgograd Plague Control Research Institute, Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing
Email: zib279@gmail.com
Researcher, Laboratory of Pathogenic Burkholderia
Russian Federation, VolgogradT.L.A. Bui
Russian-Vietnamese Tropical Research and Technology Center
Email: zib279@gmail.com
PhD (Biology), Senior Researcher, Laboratory of Molecular Biology
Viet Nam, HanoiS. Thomson
Vietnam Bear Rescue Centre
Email: zib279@gmail.com
BVSc MBA (Bachelor of Veterinary Science, Master of Business Administration), Senior Veterinary Surgeon
Viet Nam, Tam Dao National ParkI. V. Novitskaya
Volgograd Plague Control Research Institute, Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing
Email: zib279@gmail.com
PhD (Medicine), Associate Professor, Leading Researcher, Laboratory of Immunodiagnostic Preparations
Russian Federation, VolgogradReferences
- Meumann E.M., Limmathurotsakul D., Dunachie S.J., Wiersinga W.J., Currie B.J. Burkholderia pseudomallei and melioidosis. Nat. Rev. Microbiol., 2024, vol. 22, no. 3, pp. 155–169. doi: 10.1038/s41579-023-00972-5
- Chaichana P., Jenjaroen K., Amornchai P., Chumseng S., Langla S., Rongkard P., Sumonwiriya M., Jeeyapant A., Chantratita N., Teparrukkul P., Limmathurotsakul D., Day N.P.J., Wuthiekanun V., Dunachie S.J. Antibodies in melioidosis: the role of the indirect hemagglutination assay in evaluating patients and exposed populations. Am. J. Trop. Med. Hyg., 2018, vol. 99, no. 6, pp. 1378–1385. doi: 10.4269/ajtmh.17-0998
- Терешко Д.Л., Новицкая И.В., Захарова И.Б., Чиен Д., Кузнецов А.Н., Кулаков М.Я., Будченко А.А., Пушкарь В.Г., Викторов Д.В., Топорков А.В. Получение и использование эритроцитарного антигенного мелиоидозного диагностикума при анализе проб сывороток от лиц из эндемичных по мелиоидозу провинций Ха Занг, Лангшон и Куангнинь Социалистической Республики Вьетнам // Инфекция и иммунитет. 2022. Т. 12, № 5. C. 919–928. [Tereshko D.L., Novitskaya I.V., Zakharova I.B., Trien D., Kuznetsov A.N., Kulakov M.Ya., Budchenko A.A., Pushkar V.G., Viktorov D.V., Toporkov A.V. Obtaining and using erythrocyte antigenic meliidosis diagnostic agent in the analysis of serum samples from meliidosis-endemic provinces Ha Giang, Lang Son and Quang Ninh of the Socialist Republic of Vietnam. Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2022, vol. 12, no. 5, pp. 919–928. (In Russ.)] doi: 10.15789/2220-7619-OAU-1933
- Zakharova I., Teteryatnikova N., Toporkov A., Viktorov D. Development of a multiplex PCR assay for the detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei, Burkholderia thailandensis, and Burkholderia cepacia complex. Acta Trop., 2017, vol. 174, pp. 1–8. doi: 10.1016/j.actatropica.2017.06.016
- Godoy D., Randle G., Simpson A.J., Aanensen D.M., Pitt T.L., Kinoshita R., Spratt B.G. Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei. J. Clin. Microbiol., 2003, vol. 41, no. 5, pp. 2068–2079. doi: 10.1128/jcm.41.10.4913.2003
- Price E.P., MacHunter B., Spratt B.G., Wagner D.M., Currie B.J., Sarovich D.S. Improved multilocus sequence typing of Burkholderia pseudomallei and closely related species. J. Med. Microbiol., 2016, vol. 65, no. 9, pp. 992–997. doi: 10.1099/jmm.0.000312
- Lichtenegger S., Trinh T.T., Assig K., Prior K., Harmsen D., Pesl J., Zauner A., Lipp M., Que T.A., Mutsam B., Kleinhappl B., Steinmetz.I, Wagner G.E. Development and validation of a Burkholderia pseudomallei core genome multilocus sequence typing scheme to facilitate molecular surveillance. J. Clin. Microbiol., 2021, vol. 59, no. 8: e0009321. doi: 10.1128/JCM.00093-21
- Saechan V., Tongthainan D., Fungfuang W., Tulayakul P., Ieamsaard G., Ngasaman R. Natural infection of leptospirosis and melioidosis in long-tailed macaques (Macaca fascicularis) in Thailand. J. Vet. Med. Sci., 2022, vol. 84, no. 5, pp. 700–706. doi: 10.1292/jvms.21-0514
- Raj S., Sistla S., Melepurakkal Sadanandan D., Kadhiravan T., Chinnakali P. Estimation of seroprevalence of melioidosis among adult high risk groups in Southeastern India by indirect Hemagglutination assay. PLOS Glob Public Health, 2022, vol. 2, no. 5: e0000431. doi: 10.1371/journal.pgph.0000431
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